Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor

Lu Deng, Ries J. Langley, Patrick H. Brown, Gang Xu, Leslie Teng, Qian Wang, Monica I. Gonzales, Glenda G. Callender, Michael I. Nishimura, Suzanne Topalian, Roy A. Mariuzza

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Abstract

Structural studies of complexes of T cell receptor (TCR) and peptide-major histocompatibility complex (MHC) have focused on TCRs specific for foreign antigens or native self. An unexplored category of TCRs includes those specific for self determinants bearing alterations resulting from disease, notably cancer. We determined here the structure of a human melanoma-specific TCR (E8) bound to the MHC molecule HLA-DR1 and an epitope from mutant triosephosphate isomerase. The structure had features intermediate between 'anti-foreign' and autoimmune TCR-peptide-MHC class II complexes that may reflect the hybrid nature of altered self. E8 manifested very low affinity for mutant triosephosphate isomerase-HLA-DR1 despite the highly tumor-reactive properties of E8 cells. A second TCR (G4) had even lower affinity but underwent peptide-specific formation of dimers, suggesting this as a mechanism for enhancing low-affinity TCR-peptide-MHC interactions for T cell activation.

Original languageEnglish (US)
Pages (from-to)398-408
Number of pages11
JournalNature Immunology
Volume8
Issue number4
DOIs
StatePublished - Apr 2007

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ASJC Scopus subject areas

  • Immunology

Cite this

Deng, L., Langley, R. J., Brown, P. H., Xu, G., Teng, L., Wang, Q., Gonzales, M. I., Callender, G. G., Nishimura, M. I., Topalian, S., & Mariuzza, R. A. (2007). Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor. Nature Immunology, 8(4), 398-408. https://doi.org/10.1038/ni1447