Structural aspects of copper(II) binding by a multi-His analogue of somatostatin

Aleksandra Marciniak, Zaneta Czyznikowska, Marek Cebrat, Aleksandra Kotynia, Justyna Brasuń

Research output: Contribution to journalArticle

Abstract

In this paper we present the studies on coordination abilities of multi-His analogue of somatostatin. The somatostatin is a peptide hormone of which radionuclide-labeled analogues are successfully used in clinical practice in receptor scintigraphy. Its the analogue presented in this study is characterized by the presence of four His residues located in groups of two at both ends of the peptide. The -PheTrpLysThr- fragment placed between His residues is responsible for the spatial arrangement which determines the interaction with somatostatin receptors. In this paper we present the impact of copper ion binding on the spatial arrangement of the crucial fragment of peptide. The analysis of potentiometric and spectroscopic data allowed us to characterize the coordination abilities of the peptide and show that the ligand forms a {4×NIm} complex in the physiological range of pH. Results of the molecular modeling gave an insight into the structural aspects of this complex.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalInorganica Chimica Acta
Volume416
DOIs
StatePublished - May 24 2014
Externally publishedYes

Keywords

  • Coordination
  • Copper
  • Histidine
  • Potentiometricmeasurements
  • Somatostatin
  • Spectroscopicstudies

ASJC Scopus subject areas

  • Inorganic Chemistry
  • Physical and Theoretical Chemistry
  • Materials Chemistry

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    Marciniak, A., Czyznikowska, Z., Cebrat, M., Kotynia, A., & Brasuń, J. (2014). Structural aspects of copper(II) binding by a multi-His analogue of somatostatin. Inorganica Chimica Acta, 416, 57-62. https://doi.org/10.1016/j.ica.2014.03.020