Structural and functional characteristic of a model for deep-seated lacunar infarct in rats

Miroslaw Janowski, Elzbieta Gornicka-Pawlak, Hanna Kozlowska, Krystyna Domanska-Janik, Jerzy Gielecki, Barbara Lukomska

Research output: Contribution to journalArticle

Abstract

Deep-brain lacunar infarct represents a significant clinical problem as it produces severe symptoms highly resistant to rehabilitation. The limited area of necrosis may facilitate neurorepair via the action of various novel neuroprotective strategies including cell-based therapies. The lesion was induced by stereotactic injection of ouabain into adult rat brains. Subsequent behavioral testing involved beam walking task, rotarod, visual discrimination task and apomorphine rotation. For morphological and topographical analysis brain slices were stained with H-E and evaluated under light microscopy. Lesion size was measured in absolute terms and in relation to the whole brain volume. Immunohistochemical analysis for the co-localization of BrdU with specific cell-type markers (PSA-NCAM, NG2, β-tubulin III, GFAP, ED1) have has been performed, to determine the fate of newly generated cells with emphasis on evidence of neurogenesis. The lesion involved the basal ganglia, basal forebrain nuclei, internal capsule and striatum (just 1-2% of total brain volume). Significant and relatively stable behavioral deficits were observed up to 30 days. Furthermore, large numbers of cells are seen to be newly generated in response to injury with a significant proportion of these being present on account of neurogenesis.

Original languageEnglish (US)
Pages (from-to)40-48
Number of pages9
JournalJournal of the Neurological Sciences
Volume273
Issue number1-2
DOIs
StatePublished - Oct 15 2008

Keywords

  • Cell-based therapy
  • Deep-brain lesion
  • Neurogenesis
  • Neurorepair
  • Ouabain
  • Stroke

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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    Janowski, M., Gornicka-Pawlak, E., Kozlowska, H., Domanska-Janik, K., Gielecki, J., & Lukomska, B. (2008). Structural and functional characteristic of a model for deep-seated lacunar infarct in rats. Journal of the Neurological Sciences, 273(1-2), 40-48. https://doi.org/10.1016/j.jns.2008.06.019