Strong voltage-dependent inward rectification of inward rectifier K+ channels is caused by intracellular spermine

B. Fakler; U. Brändle; E. Glowatzki; S. Weidemann; H. P. Zenner; J. P. Ruppersberg

doi:10.1016/0092-8674(95)90459-X

Strong voltage-dependent inward rectification of inward rectifier K⁺ channels is caused by intracellular spermine

B. Fakler, U. Brändle, E. Glowatzki, S. Weidemann, H. P. Zenner, J. P. Ruppersberg

Research output: Contribution to journal › Article › peer-review

304 Scopus citations

Abstract

Inward rectifier K⁺ channels mediate the K⁺ conductance at resting potential in many types of cell. Since these K⁺ channels do not pass outward currents (inward rectification) when the cell membrane is depolarized beyond a trigger threshold, they play an important role in controlling excitability. Both a highly voltage-dependent block by intracellular Mg²⁺ and an endogenous gating process are presently assumed to underly inward rectification. It is shown that strong voltage dependence of rectification found under physiological conditions is predominantly due to the effect of intracellular spermine. Physiological concentrations of free spermine mediate strong rectification of IRK1 inward rectifier K⁺ channels even in the absence of free Mg²⁺ and in IRK1 mutant channels that have no endogenous rectification.

Original language	English (US)
Pages (from-to)	149-154
Number of pages	6
Journal	Cell
Volume	80
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(95)90459-X
State	Published - Jan 13 1995
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(95)90459-X

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@article{479139fda9b249e29676b02a763b6b72,

title = "Strong voltage-dependent inward rectification of inward rectifier K+ channels is caused by intracellular spermine",

abstract = "Inward rectifier K+ channels mediate the K+ conductance at resting potential in many types of cell. Since these K+ channels do not pass outward currents (inward rectification) when the cell membrane is depolarized beyond a trigger threshold, they play an important role in controlling excitability. Both a highly voltage-dependent block by intracellular Mg2+ and an endogenous gating process are presently assumed to underly inward rectification. It is shown that strong voltage dependence of rectification found under physiological conditions is predominantly due to the effect of intracellular spermine. Physiological concentrations of free spermine mediate strong rectification of IRK1 inward rectifier K+ channels even in the absence of free Mg2+ and in IRK1 mutant channels that have no endogenous rectification.",

author = "B. Fakler and U. Br{\"a}ndle and E. Glowatzki and S. Weidemann and Zenner, {H. P.} and Ruppersberg, {J. P.}",

note = "Funding Information: Correspondence should be addressed to J. P. R. We thank Dr. R. Schoepfer for the expression vector, Drs. A. W. Gummer, J. Mos-bacher, A. Busch, U. Rexhausen, and S. Waldegger for reading the manuscript, Mrs. C. KSnig for preparation of RNA, Dr. T. Doi for helpful suggestions, and Mrs. M. Ruppersberg for graphic support. This work was supported by the Deutsche Forschungsgemeinschaft (Klinische Forschergruppe H~rforschung, Ze149/6-1).",

year = "1995",

month = jan,

day = "13",

doi = "10.1016/0092-8674(95)90459-X",

language = "English (US)",

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T1 - Strong voltage-dependent inward rectification of inward rectifier K+ channels is caused by intracellular spermine

AU - Fakler, B.

AU - Brändle, U.

AU - Glowatzki, E.

AU - Weidemann, S.

AU - Zenner, H. P.

AU - Ruppersberg, J. P.

N1 - Funding Information: Correspondence should be addressed to J. P. R. We thank Dr. R. Schoepfer for the expression vector, Drs. A. W. Gummer, J. Mos-bacher, A. Busch, U. Rexhausen, and S. Waldegger for reading the manuscript, Mrs. C. KSnig for preparation of RNA, Dr. T. Doi for helpful suggestions, and Mrs. M. Ruppersberg for graphic support. This work was supported by the Deutsche Forschungsgemeinschaft (Klinische Forschergruppe H~rforschung, Ze149/6-1).

PY - 1995/1/13

Y1 - 1995/1/13

N2 - Inward rectifier K+ channels mediate the K+ conductance at resting potential in many types of cell. Since these K+ channels do not pass outward currents (inward rectification) when the cell membrane is depolarized beyond a trigger threshold, they play an important role in controlling excitability. Both a highly voltage-dependent block by intracellular Mg2+ and an endogenous gating process are presently assumed to underly inward rectification. It is shown that strong voltage dependence of rectification found under physiological conditions is predominantly due to the effect of intracellular spermine. Physiological concentrations of free spermine mediate strong rectification of IRK1 inward rectifier K+ channels even in the absence of free Mg2+ and in IRK1 mutant channels that have no endogenous rectification.

AB - Inward rectifier K+ channels mediate the K+ conductance at resting potential in many types of cell. Since these K+ channels do not pass outward currents (inward rectification) when the cell membrane is depolarized beyond a trigger threshold, they play an important role in controlling excitability. Both a highly voltage-dependent block by intracellular Mg2+ and an endogenous gating process are presently assumed to underly inward rectification. It is shown that strong voltage dependence of rectification found under physiological conditions is predominantly due to the effect of intracellular spermine. Physiological concentrations of free spermine mediate strong rectification of IRK1 inward rectifier K+ channels even in the absence of free Mg2+ and in IRK1 mutant channels that have no endogenous rectification.

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JO - Cell

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Strong voltage-dependent inward rectification of inward rectifier K⁺ channels is caused by intracellular spermine

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