Striatal microinfusion of Tourette syndrome and PANDAS sera: Failure to induce behavioral changes

Christopher R. Loiselle, Olivia Lee, Timothy H Moran, Harvey Singer

Research output: Contribution to journalArticle

Abstract

Rodent striatal microinfusions have been suggested as a model for assessing the behavioral effects induced by antineuronal antibodies. We used this approach to evaluate the proposed autoimmune etiology for Tourette syndrome (TS) and pediatric autoimmune neuropsychiatric disorders associated with streptococcal infection (PANDAS). Sera were assessed from patients with TS (n = 9) preselected based on the presence of elevated enzyme-linked immunosorbent assay optical densities against putamen homogenate and sera from patients with PANDAS (n = 8), selected from a larger group assayed for antibodies against a putamen synaptosomal preparation. The effect of antibodies against the streptococcal M5 protein were also studied. A total of 44 Fischer rats received bilateral infusion of sera: 23 ventral striatum (5 PANDAS, 5 TS, 5 anti-M5 protein, and 8 control); 21 ventrolateral striatum (5 PANDAS, 5 TS, 5 anti-M5 protein, and 6 controls). Cannulas were placed bilaterally and symmetrically by stereotactic techniques. After animals were allowed to recover for 1 week, sera were microinfused for 3 days. Animal behavior was then simultaneously quantified by daily observation and monitoring using automated activity boxes for 10 days after infusion. No significant alterations in stereotypic behavior or movement were observed between the PANDAS, TS, or anti-M5 protein and control groups. Our findings are in contrast to previous reports, and suggest the need for further investigations to determine the validity of the model and of autoimmune-mediated hypotheses for pediatric movement disorders.

Original languageEnglish (US)
Pages (from-to)390-396
Number of pages7
JournalMovement disorders : official journal of the Movement Disorder Society
Volume19
Issue number4
DOIs
StatePublished - Apr 2004

Fingerprint

Corpus Striatum
Tourette Syndrome
Serum
Putamen
Antibodies
Proteins
Stereotaxic Techniques
Animal Behavior
Inbred F344 Rats
Movement Disorders
Rodentia
Enzyme-Linked Immunosorbent Assay
Observation
Pediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal infections
Pediatrics
Control Groups

Keywords

  • Antineuronal antibodies
  • M5 protein
  • PANDAS
  • Stereotypies
  • Striatal microinfusion
  • Tourette syndrome

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Striatal microinfusion of Tourette syndrome and PANDAS sera : Failure to induce behavioral changes. / Loiselle, Christopher R.; Lee, Olivia; Moran, Timothy H; Singer, Harvey.

In: Movement disorders : official journal of the Movement Disorder Society, Vol. 19, No. 4, 04.2004, p. 390-396.

Research output: Contribution to journalArticle

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