Stress-gene induction by low-dose gamma irradiation

Albert J. Fornace, Sally A. Amundson, Khanh T. Do, Paul Meltzer, Jeffrey Trent, Michael Bittner

Research output: Contribution to journalArticlepeer-review

Abstract

Using a human myeloid tumor cell line (ML-1), we detected induction of mRNA expression of several stress-responsive genes by doses of gamma rays as low as 2 cGy. For instance, the dose response for induction of CIP1/WAF1 and GADD45 appears to be linear over the range of 2 to 50 cGy and shows no evidence of a threshold for induction. Although 2 and 5 cGy exposures did not result in any detectable reduction in cloning efficiency nor in increased apoptosis in ML-1 cells, these exposures did produce a brief cell-cycle delay. We also used fluorescent cDNA microarray hybridization to investigate transcriptional stress responses following low doses of gamma rays and to identify additional radiation-responsive genes for inclusion in a stress-specific microarray we are developing.These studies provide insight into the molecular responses to physiologically relevant doses, which cannot necessarily be extrapolated from high-dose studies. The use of high throughput arrays will allow the identification of multiple stress-responsive genes that are radiation inducible in a variety of cell types and tissues. The expectation is that transcriptional stress responses will provide a molecular approach to monitoring for radiation exposure and detecting interindividual differences.

Original languageEnglish (US)
Pages (from-to)13-15
Number of pages3
JournalMilitary medicine
Volume167
Issue number2 SUPPL.
DOIs
StatePublished - 2002

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

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