Stress and bronchodilator response in children with asthma

John M. Brehm, Sima K. Ramratnam, Sze Man Tse, Damien C. Croteau-Chonka, Maria Pino-Yanes, Christian Rosas-Salazar, Augusto A. Litonjua, Benjamin A. Raby, Nadia Boutaoui, Yueh Ying Han, Wei Chen, Erick Forno, Anna L. Marsland, Nicole R. Nugent, Celeste Eng, Angel Colón-Semidey, María Alvarez, Edna Acosta-Pérez, Melissa L. Spear, Fernando D. MartinezLydiana Avila, Scott T. Weiss, Manuel Soto-Quiros, Carole Ober, Dan L. Nicolae, Kathleen C. Barnes, Robert F. Lemanske, Robert C. Strunk, Andrew Liu, Stephanie J. London, Frank Gilliland, Patrick Sleiman, Michael March, Hakon Hakonarson, Qing Ling Duan, Jay K. Kolls, Gregory K. Fritz, Donglei Hu, Negar Fani, Jennifer S. Stevens, Lynn M. Almli, Esteban G. Burchard, Jaemin Shin, Elizabeth L. McQuaid, Kerry Ressler, Glorisa Canino, Juan C. Celedón

Research output: Contribution to journalArticle

Abstract

Rationale: Stress is associated with asthma morbidity in Puerto Ricans (PRs), who have reduced bronchodilator response (BDR). Objectives: To examine whether stress and/or a gene regulating anxiety (ADCYAP1R1) is associated with BDR in PR and non-PR children with asthma. Methods: This was a cross-sectional study of stress and BDR (percent change in FEV1 after BD) in 234 PRs ages 9-14 years with asthma. We assessed child stress using the Checklist of Children's Distress Symptoms, and maternal stress using the Perceived Stress Scale. Replication analyses were conducted in two cohorts. Polymorphisms in ADCYAP1R1 were genotyped in our study and six replication studies. Multivariable models of stress and BDR were adjusted for age, sex, income, environmental tobacco smoke, and use of inhaled corticosteroids. Measurements and Main Results: High child stress was associated with reduced BDR in three cohorts. PR children who were highly stressed (upper quartile, Checklist of Children's Distress Symptoms) and whose mothers had high stress (upper quartile, Perceived Stress Scale) had a BDR that was 10.2% (95% confidence interval, 6.1-14.2%) lower than children who had neither high stress nor a highly stressed mother. A polymorphism in ADCYAP1R1 (rs34548976) was associated with reduced BDR. This single-nucleotide polymorphism is associated with reduced expression of the gene for the β2-adrenergic receptor (ADRB2) in CD4+ lymphocytes of subjects with asthma, and it affects brain connectivity of the amygdala and the insula (a biomarker of anxiety). Conclusions: High child stress and an ADCYAP1R1 singlenucleotide polymorphism are associated with reduced BDR in children with asthma. This is likely caused by down-regulation of ADRB2 in highly stressed children.

Original languageEnglish (US)
Pages (from-to)47-56
Number of pages10
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume192
Issue number1
DOIs
StatePublished - Jul 1 2015

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Bronchodilator Agents
Asthma
Hispanic Americans
Mothers
Checklist
Anxiety
Tobacco Use
Amygdala
Smoke
Adrenergic Receptors
Single Nucleotide Polymorphism
Adrenal Cortex Hormones
Down-Regulation
Cross-Sectional Studies
Biomarkers
Lymphocytes
Confidence Intervals
Morbidity
Gene Expression

Keywords

  • Asthma
  • Bronchodilator response
  • Puerto ricans
  • Stress

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Brehm, J. M., Ramratnam, S. K., Tse, S. M., Croteau-Chonka, D. C., Pino-Yanes, M., Rosas-Salazar, C., ... Celedón, J. C. (2015). Stress and bronchodilator response in children with asthma. American Journal of Respiratory and Critical Care Medicine, 192(1), 47-56. https://doi.org/10.1164/rccm.201501-0037OC

Stress and bronchodilator response in children with asthma. / Brehm, John M.; Ramratnam, Sima K.; Tse, Sze Man; Croteau-Chonka, Damien C.; Pino-Yanes, Maria; Rosas-Salazar, Christian; Litonjua, Augusto A.; Raby, Benjamin A.; Boutaoui, Nadia; Han, Yueh Ying; Chen, Wei; Forno, Erick; Marsland, Anna L.; Nugent, Nicole R.; Eng, Celeste; Colón-Semidey, Angel; Alvarez, María; Acosta-Pérez, Edna; Spear, Melissa L.; Martinez, Fernando D.; Avila, Lydiana; Weiss, Scott T.; Soto-Quiros, Manuel; Ober, Carole; Nicolae, Dan L.; Barnes, Kathleen C.; Lemanske, Robert F.; Strunk, Robert C.; Liu, Andrew; London, Stephanie J.; Gilliland, Frank; Sleiman, Patrick; March, Michael; Hakonarson, Hakon; Duan, Qing Ling; Kolls, Jay K.; Fritz, Gregory K.; Hu, Donglei; Fani, Negar; Stevens, Jennifer S.; Almli, Lynn M.; Burchard, Esteban G.; Shin, Jaemin; McQuaid, Elizabeth L.; Ressler, Kerry; Canino, Glorisa; Celedón, Juan C.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 192, No. 1, 01.07.2015, p. 47-56.

Research output: Contribution to journalArticle

Brehm, JM, Ramratnam, SK, Tse, SM, Croteau-Chonka, DC, Pino-Yanes, M, Rosas-Salazar, C, Litonjua, AA, Raby, BA, Boutaoui, N, Han, YY, Chen, W, Forno, E, Marsland, AL, Nugent, NR, Eng, C, Colón-Semidey, A, Alvarez, M, Acosta-Pérez, E, Spear, ML, Martinez, FD, Avila, L, Weiss, ST, Soto-Quiros, M, Ober, C, Nicolae, DL, Barnes, KC, Lemanske, RF, Strunk, RC, Liu, A, London, SJ, Gilliland, F, Sleiman, P, March, M, Hakonarson, H, Duan, QL, Kolls, JK, Fritz, GK, Hu, D, Fani, N, Stevens, JS, Almli, LM, Burchard, EG, Shin, J, McQuaid, EL, Ressler, K, Canino, G & Celedón, JC 2015, 'Stress and bronchodilator response in children with asthma', American Journal of Respiratory and Critical Care Medicine, vol. 192, no. 1, pp. 47-56. https://doi.org/10.1164/rccm.201501-0037OC
Brehm JM, Ramratnam SK, Tse SM, Croteau-Chonka DC, Pino-Yanes M, Rosas-Salazar C et al. Stress and bronchodilator response in children with asthma. American Journal of Respiratory and Critical Care Medicine. 2015 Jul 1;192(1):47-56. https://doi.org/10.1164/rccm.201501-0037OC
Brehm, John M. ; Ramratnam, Sima K. ; Tse, Sze Man ; Croteau-Chonka, Damien C. ; Pino-Yanes, Maria ; Rosas-Salazar, Christian ; Litonjua, Augusto A. ; Raby, Benjamin A. ; Boutaoui, Nadia ; Han, Yueh Ying ; Chen, Wei ; Forno, Erick ; Marsland, Anna L. ; Nugent, Nicole R. ; Eng, Celeste ; Colón-Semidey, Angel ; Alvarez, María ; Acosta-Pérez, Edna ; Spear, Melissa L. ; Martinez, Fernando D. ; Avila, Lydiana ; Weiss, Scott T. ; Soto-Quiros, Manuel ; Ober, Carole ; Nicolae, Dan L. ; Barnes, Kathleen C. ; Lemanske, Robert F. ; Strunk, Robert C. ; Liu, Andrew ; London, Stephanie J. ; Gilliland, Frank ; Sleiman, Patrick ; March, Michael ; Hakonarson, Hakon ; Duan, Qing Ling ; Kolls, Jay K. ; Fritz, Gregory K. ; Hu, Donglei ; Fani, Negar ; Stevens, Jennifer S. ; Almli, Lynn M. ; Burchard, Esteban G. ; Shin, Jaemin ; McQuaid, Elizabeth L. ; Ressler, Kerry ; Canino, Glorisa ; Celedón, Juan C. / Stress and bronchodilator response in children with asthma. In: American Journal of Respiratory and Critical Care Medicine. 2015 ; Vol. 192, No. 1. pp. 47-56.
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abstract = "Rationale: Stress is associated with asthma morbidity in Puerto Ricans (PRs), who have reduced bronchodilator response (BDR). Objectives: To examine whether stress and/or a gene regulating anxiety (ADCYAP1R1) is associated with BDR in PR and non-PR children with asthma. Methods: This was a cross-sectional study of stress and BDR (percent change in FEV1 after BD) in 234 PRs ages 9-14 years with asthma. We assessed child stress using the Checklist of Children's Distress Symptoms, and maternal stress using the Perceived Stress Scale. Replication analyses were conducted in two cohorts. Polymorphisms in ADCYAP1R1 were genotyped in our study and six replication studies. Multivariable models of stress and BDR were adjusted for age, sex, income, environmental tobacco smoke, and use of inhaled corticosteroids. Measurements and Main Results: High child stress was associated with reduced BDR in three cohorts. PR children who were highly stressed (upper quartile, Checklist of Children's Distress Symptoms) and whose mothers had high stress (upper quartile, Perceived Stress Scale) had a BDR that was 10.2{\%} (95{\%} confidence interval, 6.1-14.2{\%}) lower than children who had neither high stress nor a highly stressed mother. A polymorphism in ADCYAP1R1 (rs34548976) was associated with reduced BDR. This single-nucleotide polymorphism is associated with reduced expression of the gene for the β2-adrenergic receptor (ADRB2) in CD4+ lymphocytes of subjects with asthma, and it affects brain connectivity of the amygdala and the insula (a biomarker of anxiety). Conclusions: High child stress and an ADCYAP1R1 singlenucleotide polymorphism are associated with reduced BDR in children with asthma. This is likely caused by down-regulation of ADRB2 in highly stressed children.",
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T1 - Stress and bronchodilator response in children with asthma

AU - Brehm, John M.

AU - Ramratnam, Sima K.

AU - Tse, Sze Man

AU - Croteau-Chonka, Damien C.

AU - Pino-Yanes, Maria

AU - Rosas-Salazar, Christian

AU - Litonjua, Augusto A.

AU - Raby, Benjamin A.

AU - Boutaoui, Nadia

AU - Han, Yueh Ying

AU - Chen, Wei

AU - Forno, Erick

AU - Marsland, Anna L.

AU - Nugent, Nicole R.

AU - Eng, Celeste

AU - Colón-Semidey, Angel

AU - Alvarez, María

AU - Acosta-Pérez, Edna

AU - Spear, Melissa L.

AU - Martinez, Fernando D.

AU - Avila, Lydiana

AU - Weiss, Scott T.

AU - Soto-Quiros, Manuel

AU - Ober, Carole

AU - Nicolae, Dan L.

AU - Barnes, Kathleen C.

AU - Lemanske, Robert F.

AU - Strunk, Robert C.

AU - Liu, Andrew

AU - London, Stephanie J.

AU - Gilliland, Frank

AU - Sleiman, Patrick

AU - March, Michael

AU - Hakonarson, Hakon

AU - Duan, Qing Ling

AU - Kolls, Jay K.

AU - Fritz, Gregory K.

AU - Hu, Donglei

AU - Fani, Negar

AU - Stevens, Jennifer S.

AU - Almli, Lynn M.

AU - Burchard, Esteban G.

AU - Shin, Jaemin

AU - McQuaid, Elizabeth L.

AU - Ressler, Kerry

AU - Canino, Glorisa

AU - Celedón, Juan C.

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Rationale: Stress is associated with asthma morbidity in Puerto Ricans (PRs), who have reduced bronchodilator response (BDR). Objectives: To examine whether stress and/or a gene regulating anxiety (ADCYAP1R1) is associated with BDR in PR and non-PR children with asthma. Methods: This was a cross-sectional study of stress and BDR (percent change in FEV1 after BD) in 234 PRs ages 9-14 years with asthma. We assessed child stress using the Checklist of Children's Distress Symptoms, and maternal stress using the Perceived Stress Scale. Replication analyses were conducted in two cohorts. Polymorphisms in ADCYAP1R1 were genotyped in our study and six replication studies. Multivariable models of stress and BDR were adjusted for age, sex, income, environmental tobacco smoke, and use of inhaled corticosteroids. Measurements and Main Results: High child stress was associated with reduced BDR in three cohorts. PR children who were highly stressed (upper quartile, Checklist of Children's Distress Symptoms) and whose mothers had high stress (upper quartile, Perceived Stress Scale) had a BDR that was 10.2% (95% confidence interval, 6.1-14.2%) lower than children who had neither high stress nor a highly stressed mother. A polymorphism in ADCYAP1R1 (rs34548976) was associated with reduced BDR. This single-nucleotide polymorphism is associated with reduced expression of the gene for the β2-adrenergic receptor (ADRB2) in CD4+ lymphocytes of subjects with asthma, and it affects brain connectivity of the amygdala and the insula (a biomarker of anxiety). Conclusions: High child stress and an ADCYAP1R1 singlenucleotide polymorphism are associated with reduced BDR in children with asthma. This is likely caused by down-regulation of ADRB2 in highly stressed children.

AB - Rationale: Stress is associated with asthma morbidity in Puerto Ricans (PRs), who have reduced bronchodilator response (BDR). Objectives: To examine whether stress and/or a gene regulating anxiety (ADCYAP1R1) is associated with BDR in PR and non-PR children with asthma. Methods: This was a cross-sectional study of stress and BDR (percent change in FEV1 after BD) in 234 PRs ages 9-14 years with asthma. We assessed child stress using the Checklist of Children's Distress Symptoms, and maternal stress using the Perceived Stress Scale. Replication analyses were conducted in two cohorts. Polymorphisms in ADCYAP1R1 were genotyped in our study and six replication studies. Multivariable models of stress and BDR were adjusted for age, sex, income, environmental tobacco smoke, and use of inhaled corticosteroids. Measurements and Main Results: High child stress was associated with reduced BDR in three cohorts. PR children who were highly stressed (upper quartile, Checklist of Children's Distress Symptoms) and whose mothers had high stress (upper quartile, Perceived Stress Scale) had a BDR that was 10.2% (95% confidence interval, 6.1-14.2%) lower than children who had neither high stress nor a highly stressed mother. A polymorphism in ADCYAP1R1 (rs34548976) was associated with reduced BDR. This single-nucleotide polymorphism is associated with reduced expression of the gene for the β2-adrenergic receptor (ADRB2) in CD4+ lymphocytes of subjects with asthma, and it affects brain connectivity of the amygdala and the insula (a biomarker of anxiety). Conclusions: High child stress and an ADCYAP1R1 singlenucleotide polymorphism are associated with reduced BDR in children with asthma. This is likely caused by down-regulation of ADRB2 in highly stressed children.

KW - Asthma

KW - Bronchodilator response

KW - Puerto ricans

KW - Stress

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