Streptococcus mitis Expressing Pneumococcal Serotype 1 Capsule

Fernanda C. Lessa, Jennifer Milucky, Nadine G. Rouphael, Nancy M. Bennett, H. Keipp Talbot, Lee H. Harrison, Monica M. Farley, Jeremy Walston, Fabiana Pimenta, Robert E. Gertz, Gowrisankar Rajam, Maria da Gloria Carvalho, Bernard Beall, Cynthia G. Whitney

Research output: Contribution to journalArticlepeer-review

Abstract

Streptococcus pneumoniae’s polysaccharide capsule is an important virulence factor; vaccine-induced immunity to specific capsular polysaccharide effectively prevents disease. Serotype 1 S. pneumoniae is rarely found in healthy persons, but is highly invasive and a common cause of meningitis outbreaks and invasive disease outside of the United States. Here we show that genes for polysaccharide capsule similar to those expressed by pneumococci were commonly detected by polymerase chain reaction among upper respiratory tract samples from older US adults not carrying pneumococci. Serotype 1-specific genes were predominantly detected. In five oropharyngeal samples tested, serotype 1 gene belonging to S. mitis expressed capsules immunologically indistinct from pneumococcal capsules. Whole genome sequencing revealed three distinct S. mitis clones, each representing a cps1 operon highly similar to the pneumococcal cps1 reference operon. These findings raise important questions about the contribution of commensal streptococci to natural immunity against pneumococci, a leading cause of mortality worldwide.

Original languageEnglish (US)
Article number17959
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

ASJC Scopus subject areas

  • General

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