Strategy for epitope tagging the protein-coding region of any gene

P. Surdej; M. Jacobs-Lorena

Strategy for epitope tagging the protein-coding region of any gene

P. Surdej, M. Jacobs-Lorena

Research output: Contribution to journal › Article › peer-review

Abstract

We describe the construction of a plasmid carrying a DNA cassette encoding the influenza virus hemagglutinin (HAI) epitope. Insertion of this cassette into the coding region creates a gene encoding an epitope-tagged protein. The tagged protein can be detected and quantified by using a commercially available anti-HAI antibody. A one-step procedure introduces the epitope at any restriction site within a gene while maintaining the open reading frame. An example of the application of this technique is presented and the different uses of the cassette are discussed.

Original language	English (US)
Pages (from-to)	560-562+564
Journal	BioTechniques
Volume	17
Issue number	3
State	Published - Jan 1 1994
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
General Biochemistry, Genetics and Molecular Biology

Cite this

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Strategy for epitope tagging the protein-coding region of any gene

Abstract

ASJC Scopus subject areas

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