Strategy for epitope tagging the protein-coding region of any gene

P. Surdej, M. Jacobs-Lorena

Research output: Contribution to journalArticle

Abstract

We describe the construction of a plasmid carrying a DNA cassette encoding the influenza virus hemagglutinin (HAI) epitope. Insertion of this cassette into the coding region creates a gene encoding an epitope-tagged protein. The tagged protein can be detected and quantified by using a commercially available anti-HAI antibody. A one-step procedure introduces the epitope at any restriction site within a gene while maintaining the open reading frame. An example of the application of this technique is presented and the different uses of the cassette are discussed.

Original languageEnglish (US)
Pages (from-to)560-562+564
JournalBioTechniques
Volume17
Issue number3
StatePublished - Jan 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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