Strain hardening of actin filament networks: Regulation by the dynamic cross-linking protein α-actinin

J. Xu, Y. Tseng, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

Mechanical stresses applied to the plasma membrane of an adherent cell induces strain hardening of the cytoskeleton, i.e. the elasticity of the cytoskeleton increases with its deformation. Strain hardening is thought to mediate the transduction of mechanical signals across the plasma membrane through the cytoskeleton. Here, we describe the strain dependence of a model system consisting of actin filaments (F-actin), a major component of the cytoskeleton, and the F-actin cross-linking protein α-actinin, which localizes along contractile stress fibers and at focal adhesions. We show that the amplitude and rate of shear deformations regulate the resilience of F-actin networks. At low temperatures, for which the lifetime of binding of α-actinin to F-actin is long, F-actin/α-actinin networks exhibit strong strain hardening at short time scales and soften at long time scales. For F-actin networks in the absence of α-actinin or for F-actin/α-actinin networks at high temperatures, strain hardening appears only at very short time scales. We propose a model of strain hardening for F-actin networks, based on both the intrinsic rigidity of F-actin and dynamic topological constraints formed by the cross-linkers located at filaments entanglements. This model offers an explanation for the origin of strain hardening observed when shear stresses are applied against the cellular membrane.

Original languageEnglish (US)
Pages (from-to)35886-35892
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number46
DOIs
StatePublished - Nov 17 2000

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Actinin
Actin Cytoskeleton
Strain hardening
Actins
Cytoskeleton
Proteins
Cell membranes
Cellular Mechanotransduction
Cell Membrane
Stress Fibers
Mechanical Stress
Temperature
Focal Adhesions
Elasticity
Rigidity
Shear deformation
Shear stress
Adhesion
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Strain hardening of actin filament networks : Regulation by the dynamic cross-linking protein α-actinin. / Xu, J.; Tseng, Y.; Wirtz, Denis.

In: Journal of Biological Chemistry, Vol. 275, No. 46, 17.11.2000, p. 35886-35892.

Research output: Contribution to journalArticle

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