Strain differences in neointimal hyperplasia in the rat

Shahin Assadnia, John P. Rapp, Andrea L. Nestor, Timothy Pringle, Greg J. Cerilli, William T. Gunning, Thomas H. Webb, Mark Kligman, David C. Allison

Research output: Contribution to journalArticle

Abstract

We performed an initial screen of 11 rat strains by use of a standard balloon injury to the left iliac artery to observe whether genetically determined differences existed in the development of neointimal hyperplasia. Neointimal hyperplasia was assayed 8 weeks after the vascular injury on coded microscopic sections. Statistically significant differences in the percentages of the vascular wall cross-sectional areas composed of intima (percentage intima) secondary to neointimal hyperplasia were noted among the different rat strains (P<0.02), with the Brown-Norway (BN), Dark Agouti, and Milan normotensive strain rats having the highest and the spontaneously hypertensive rats (SHR) having the lowest percentages of intima. In a separate experiment, F1 hybrids of SHR X BN strains and parental BN and SHR underwent the vascular injury, and the parental strains again showed a statistically significant difference from one another in the mean percentage of intima (P<0.0001). The F1 hybrids showed an average percentage of intima intermediate between those of the parental strains. The average lumen size of the injured BN vessels were significantly smaller than that of the noninjured control vessels (P=0.044), but this significance disappeared when the circular areas of these vessels were calculated without taking neointimal growth into consideration (P=0.649). These results provide the groundwork for a genetic linkage analysis to identify the genes that influence the development of neointimal hyperplasia after vascular injury.

Original languageEnglish (US)
Pages (from-to)1252-1257
Number of pages6
JournalCirculation research
Volume84
Issue number11
DOIs
StatePublished - Jun 11 1999

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Keywords

  • Balloon injury
  • Neointimal hyperplasia
  • Rat, inbred strain
  • Restenosis

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Assadnia, S., Rapp, J. P., Nestor, A. L., Pringle, T., Cerilli, G. J., Gunning, W. T., Webb, T. H., Kligman, M., & Allison, D. C. (1999). Strain differences in neointimal hyperplasia in the rat. Circulation research, 84(11), 1252-1257. https://doi.org/10.1161/01.RES.84.11.1252