Strain differences in myenteric neuron number and CCK1 receptor mRNA expression may account for differences in CCK induced c-Fos activation

Stephen Gulley, Sanjay K. Sharma, Mahmoud Mansour, Cherese N. Sullivan, Timothy H Moran, Ayman I. Sayegh

Research output: Contribution to journalArticle

Abstract

We utilized a diaminobenzidine reaction enhanced with nickel to compare dorsal vagal complex (DVC) and myenteric neuronal Fos-Like immunoreactivity (Fos-LI), in response to sulfated cholecystokinin-8 (CCK-8) (5, 10, 20, 40 μg/kg), among Sprague-Dawley (SD), Standard Long-Evans (SLE), Otsuka Long-Evans Tokushima Fatty (OLETF), and Long-Evans Tokushima Otsuka (LETO) rats. All rat strains but OLETF expressed Fos-LI in response to CCK-8. In addition, SD rats expressed more Fos-LI in the area postrema and myenteric neurons than SLE and LETO rats. To investigate the basis for these differences, we utilized cuprolinic blue staining, which stains neuronal cell bodies, to quantify the number of myenteric neurons, and a reverse transcriptase chain polymerase reaction to measure the gene expression of CCK1 receptor in the gut. We found that SD rats have significantly more duodenal myenteric neurons than the other strains. In addition, this strain expressed significantly higher levels of the CCK1 gene in both the duodenum and jejunum than the other strains. In conclusion, SD rats may express more myenteric Fos-LI in response to CCK due to increased numbers of myenteric neurons or more intestinal CCK1 receptors than the other strains of rats.

Original languageEnglish (US)
Pages (from-to)109-119
Number of pages11
JournalBrain Research
Volume1058
Issue number1-2
DOIs
StatePublished - Oct 5 2005

Fingerprint

Inbred OLETF Rats
Sprague Dawley Rats
Neurons
Messenger RNA
Area Postrema
Jejunum
Nickel
Reverse Transcriptase Polymerase Chain Reaction
Duodenum
Coloring Agents
Staining and Labeling
Gene Expression
Genes
cholecystokinin 8

Keywords

  • Area postrema
  • CCK receptor
  • Cholecystokinin
  • Dorsal vagal complex
  • Fos
  • Immunohistochemistry
  • LETO
  • Long-Evans
  • Myenteric plexus
  • NTS
  • OLETF
  • Sprague-Dawley

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Strain differences in myenteric neuron number and CCK1 receptor mRNA expression may account for differences in CCK induced c-Fos activation. / Gulley, Stephen; Sharma, Sanjay K.; Mansour, Mahmoud; Sullivan, Cherese N.; Moran, Timothy H; Sayegh, Ayman I.

In: Brain Research, Vol. 1058, No. 1-2, 05.10.2005, p. 109-119.

Research output: Contribution to journalArticle

Gulley, Stephen ; Sharma, Sanjay K. ; Mansour, Mahmoud ; Sullivan, Cherese N. ; Moran, Timothy H ; Sayegh, Ayman I. / Strain differences in myenteric neuron number and CCK1 receptor mRNA expression may account for differences in CCK induced c-Fos activation. In: Brain Research. 2005 ; Vol. 1058, No. 1-2. pp. 109-119.
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AU - Sullivan, Cherese N.

AU - Moran, Timothy H

AU - Sayegh, Ayman I.

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AB - We utilized a diaminobenzidine reaction enhanced with nickel to compare dorsal vagal complex (DVC) and myenteric neuronal Fos-Like immunoreactivity (Fos-LI), in response to sulfated cholecystokinin-8 (CCK-8) (5, 10, 20, 40 μg/kg), among Sprague-Dawley (SD), Standard Long-Evans (SLE), Otsuka Long-Evans Tokushima Fatty (OLETF), and Long-Evans Tokushima Otsuka (LETO) rats. All rat strains but OLETF expressed Fos-LI in response to CCK-8. In addition, SD rats expressed more Fos-LI in the area postrema and myenteric neurons than SLE and LETO rats. To investigate the basis for these differences, we utilized cuprolinic blue staining, which stains neuronal cell bodies, to quantify the number of myenteric neurons, and a reverse transcriptase chain polymerase reaction to measure the gene expression of CCK1 receptor in the gut. We found that SD rats have significantly more duodenal myenteric neurons than the other strains. In addition, this strain expressed significantly higher levels of the CCK1 gene in both the duodenum and jejunum than the other strains. In conclusion, SD rats may express more myenteric Fos-LI in response to CCK due to increased numbers of myenteric neurons or more intestinal CCK1 receptors than the other strains of rats.

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