Strain construction and screening methods for a yeast histone H3/H4 mutant library

Junbiao Dai, Jef D. Boeke

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A mutant library consisting of hundreds of designed point and deletion mutants in the genes encoding Saccharomyces cerevisiae histones H3 and H4 is described. Incorporation of this library into a suitably engineered yeast strain (e.g., bearing a reporter of interest), and the validation of individual library members is described in detail.

Original languageEnglish (US)
Title of host publicationChromatin Remodeling
Subtitle of host publicationMethods and Protocols
EditorsRandall H. Morse
Pages1-14
Number of pages14
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume833
ISSN (Print)1064-3745

Keywords

  • Chromatin remodeling
  • High-throughput mutagenesis
  • Histones modification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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