TY - JOUR
T1 - Storage and secretion of Ag-Aper14, a novel peritrophic matrix protein, and Ag-Muc1 from the mosquito Anopheles gambiae
AU - Devenport, M.
AU - Fujioka, H.
AU - Donnelly-Doman, M.
AU - Shen, Z.
AU - Jacobs-Lorena, M.
N1 - Funding Information:
This work was supported by grants from the National Institutes of Health. Confocal microscopy research was supported by CWRU Ireland Comprehensive Cancer Center departmental grant P30 CA43703-12.
PY - 2005/4
Y1 - 2005/4
N2 - The gene Ag-Aper14 encodes a novel peritrophic matrix (or peritrophic membrane; PM) protein in the mosquito Anopheles gambiae. The Ag-Aper14 protein is merely 89 amino acids long and has a single putative chitin-binding domain. Prior to blood feeding, the Ag-Aper14 protein is stored in secretory vesicles next to the epithelial cell lumenal surface. Immunoelectron microscopy has revealed that Ag-Aper14 co-localizes to the same secretory vesicles as another PM protein, Ag-Aper1, indicating a common mode of regulated secretion. Conversely, Ag-Muc1, an epithelial cell-surface protein, does not co-localize to these secretory vesicles and is detected only on the cell surface. After blood feeding, Ag-Aper14 is secreted and incorporated into the PM that surrounds the ingested blood.
AB - The gene Ag-Aper14 encodes a novel peritrophic matrix (or peritrophic membrane; PM) protein in the mosquito Anopheles gambiae. The Ag-Aper14 protein is merely 89 amino acids long and has a single putative chitin-binding domain. Prior to blood feeding, the Ag-Aper14 protein is stored in secretory vesicles next to the epithelial cell lumenal surface. Immunoelectron microscopy has revealed that Ag-Aper14 co-localizes to the same secretory vesicles as another PM protein, Ag-Aper1, indicating a common mode of regulated secretion. Conversely, Ag-Muc1, an epithelial cell-surface protein, does not co-localize to these secretory vesicles and is detected only on the cell surface. After blood feeding, Ag-Aper14 is secreted and incorporated into the PM that surrounds the ingested blood.
KW - Midgut
KW - Mosquito Anopheles gambiae (Insecta)
KW - Mucin
KW - Peritrophic matrix
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U2 - 10.1007/s00441-004-1067-3
DO - 10.1007/s00441-004-1067-3
M3 - Article
C2 - 15726420
AN - SCOPUS:17444401719
VL - 320
SP - 175
EP - 185
JO - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)
JF - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)
SN - 0302-766X
IS - 1
ER -