Stimulus-induced pacemaker activity in interstitial cells of Cajal associated with the deep muscular plexus of the small intestine

Yaohui Zhu, X. Y. Wang, S. P. Parsons, J. D. Huizinga

Research output: Contribution to journalArticle


Background: The ICC-DMP have been proposed to generate stimulus-dependent pacemaker activity, rhythmic transient depolarizations, that take part in orchestrating segmentation and clustered propulsive motor patterns in the small intestine. However, little is known about the fundamental properties of ICC-DMP. Methods: This study was undertaken to increase our understanding of intrinsic properties of the ICC-DMP through calcium imaging and intracellular electrical recordings. Key Results: Without stimulation, most ICC-DMP were quiescent. In some preparations ICC-DMP generated rhythmic low-frequency calcium oscillations (35 cpm). Immunohistochemistry proved the existence of NK1R on the ICC-DMP and close contacts between ICC-DMP and substance P-positive nerves. Substance P (25 nM) induced low-frequency calcium oscillations that were synchronized across the ICC-DMP network. Substance P also induced low frequency rhythmic transient depolarizations (

Original languageEnglish (US)
Pages (from-to)1064-1074
Number of pages11
JournalNeurogastroenterology and Motility
Issue number7
StatePublished - Jul 1 2016
Externally publishedYes



  • calcium transients
  • interstitial cells of Cajal
  • intestinal motility
  • pacemaker activity
  • propulsion
  • segmentation
  • substance P

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Gastroenterology
  • Physiology

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