Stimulation of rat sertoli cell adenylate cyclase by germ cells in vitro

M. J. Welsh, M. E. Ireland, G. J. Treisman

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of germ cells or germ cell fractions on adenylate cyclase (AC) activity in membrane preparations from cultured rat Sertoli cells has been examined. Whole germ cells or 30,000 X g pellet or supernatant fractions of germ cells have the ability to stimulate Sertoli cell AC to levels comparable to those measured in follicle-stimulating hormone-stimulated Sertoli cell membranes. Treatment at 100°C but not 60°C for 1 min abolished the ability of germ cell preparations to stimulate Sertoli AC. Germ cell stimulation of Sertoli cell AC was not calcium dependent, was not blocked by propranolol, and was observed to be dose dependent.

Original languageEnglish (US)
Pages (from-to)1050-1056
Number of pages7
JournalBiology of reproduction
Volume33
Issue number5
DOIs
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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