Stimulation of Opioid receptors on Cardiac Ventricular Myocytes reduces L Type Ca2+ Channel Current

Rui Ping Xiao, Harold A. Spurgeon, Maurizio C. Capogrossi, Edward Lakatta

Research output: Contribution to journalArticle

Abstract

Recent studies have indicated that opioid peptide receptors are present on cardiac ventricular cells and that Leucine enkephalin (LE), a naturally occurring δ opioid peptide receptor agonist, leads to marked reductions in twitch amplitude and in the cytosolic Ca2+ transient (Cai) of single adult rat ventricular myocytes. The specific mechanism by which Cai is reduced by LE have not been fully elucidated. Specifically, it is unknown whether LE affects the Ca2+ current (ICa) of L type Ca2+ channels. In the present study we determined the effect of LE on ICa of individual cardiac ventricular cells freshly isolated from adult rats. LE (10-8M) decreased the amplitude of ICa by 40% (during regular whole cell voltage clamp depolarizations to 0 mV at 0.5 Hz at 23°C from a holding potential of -40 mV). The relative magnitude of this effect increased with the magnitude of the test potential from -20 to +50 mV. ICa inactivation was also prolonged by LE. These effects of LE on ICa were abolished by Naloxone (NAL), an opioid receptor antagonist. Thus, the effects of the opioid peptide, LE, to decrease the Cai transient and contraction amplitudes in individual cardiac ventricular cells, are, in part, mediated by an LE induced reduction in ICa .

Original languageEnglish (US)
Pages (from-to)661-666
Number of pages6
JournalJournal of Molecular and Cellular Cardiology
Volume25
Issue number6
DOIs
StatePublished - Jun 1993
Externally publishedYes

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Leucine Enkephalin
Opioid Receptors
Cardiac Myocytes
Opioid Peptides
Peptide Receptors
Narcotic Antagonists
Muscle Cells

Keywords

  • Action potential
  • Calcium current
  • Cardiac cells
  • Opioid receptors

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Stimulation of Opioid receptors on Cardiac Ventricular Myocytes reduces L Type Ca2+ Channel Current. / Xiao, Rui Ping; Spurgeon, Harold A.; Capogrossi, Maurizio C.; Lakatta, Edward.

In: Journal of Molecular and Cellular Cardiology, Vol. 25, No. 6, 06.1993, p. 661-666.

Research output: Contribution to journalArticle

Xiao, Rui Ping ; Spurgeon, Harold A. ; Capogrossi, Maurizio C. ; Lakatta, Edward. / Stimulation of Opioid receptors on Cardiac Ventricular Myocytes reduces L Type Ca2+ Channel Current. In: Journal of Molecular and Cellular Cardiology. 1993 ; Vol. 25, No. 6. pp. 661-666.
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