Stimulation of endogenous endonuclease activity in hepatocytes exposed to oxidative stress

David J. McConkey, Pia Hartzell, Pierluigi Nicotera, Andrew H. Wyllie, Sten Orrenius

Research output: Contribution to journalArticlepeer-review

Abstract

Incubation of freshly isolated rat hepatocytes with moderately toxic concentrations of menadione [2-methyl-1,4-naphthoquinone) resulted in chromatin condensation and progressive DNA fragmentation, suggestive of the stimulation of an endogenous endonuclease activity previously found to be involved in programmed cell death (apoptosis). Endonuclease activation followed upon a sustained increase in cytosolic Ca2+ concentration and preceded cell killing by 1-2 h. It is concluded that generation of oxidative stress in hepatocytes can activate processes similar to those observed during programmed cell death.

Original languageEnglish (US)
Pages (from-to)123-130
Number of pages8
JournalToxicology Letters
Volume42
Issue number2
DOIs
StatePublished - Aug 1988
Externally publishedYes

Keywords

  • Calcium
  • DNA fragmentation
  • Isolated hepatocytes
  • Menadione

ASJC Scopus subject areas

  • Toxicology

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