Stimulation-associated redistribution of Na,K-ATPase in rat lacrimal gland

Samuel Chi-Hung Yiu, Ross W. Lambert, Michael E. Bradley, Claire E. Ingham, Klane L. Hales, Richard L. Wood, Austin K. Mircheff

Research output: Contribution to journalArticle

Abstract

To test the possibility that stimulation of secretion leads Na,K-ATPase to be recruited from cytoplasmic pools and inserted into basal-lateral plasma membranes, we surveyed the subcellular distributions of Na, K-ATPase in resting and stimulated fragments of rat exorbital lacrimal gland. After a two-dimensional separation procedure based on differential sedimentation and density gradient centrifugation, we defined six density windows, which differ from one another in their contents of biochemical markers. The membranes equilibrating in window I could be identified as a sample of basal-lateral membranes; in resting preparations these membranes contained Na,K-ATPase enriched 16.6-fold with respect to the initial homogenates. Windows II through VI contained various cytoplasmic membrane populations; these accounted for roughly 80% of the total recovered Na,K-ATPase activity. Thirty-minute stimulation with 10 μm carbachol caused a 1.4-fold increase (P

Original languageEnglish (US)
Pages (from-to)185-194
Number of pages10
JournalJournal of Membrane Biology
Volume102
Issue number3
DOIs
StatePublished - Jun 1988
Externally publishedYes

Fingerprint

Lacrimal Apparatus
Membranes
Cell Membrane
Density Gradient Centrifugation
Carbachol
Biomarkers
sodium-translocating ATPase
Population

Keywords

  • cell volume regulation
  • exocrine glands
  • fluid and electrolyte secretion
  • Golgi complex
  • membrane recycling
  • stimulus-secretion coupling

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

Cite this

Yiu, S. C-H., Lambert, R. W., Bradley, M. E., Ingham, C. E., Hales, K. L., Wood, R. L., & Mircheff, A. K. (1988). Stimulation-associated redistribution of Na,K-ATPase in rat lacrimal gland. Journal of Membrane Biology, 102(3), 185-194. https://doi.org/10.1007/BF01925712

Stimulation-associated redistribution of Na,K-ATPase in rat lacrimal gland. / Yiu, Samuel Chi-Hung; Lambert, Ross W.; Bradley, Michael E.; Ingham, Claire E.; Hales, Klane L.; Wood, Richard L.; Mircheff, Austin K.

In: Journal of Membrane Biology, Vol. 102, No. 3, 06.1988, p. 185-194.

Research output: Contribution to journalArticle

Yiu, SC-H, Lambert, RW, Bradley, ME, Ingham, CE, Hales, KL, Wood, RL & Mircheff, AK 1988, 'Stimulation-associated redistribution of Na,K-ATPase in rat lacrimal gland', Journal of Membrane Biology, vol. 102, no. 3, pp. 185-194. https://doi.org/10.1007/BF01925712
Yiu, Samuel Chi-Hung ; Lambert, Ross W. ; Bradley, Michael E. ; Ingham, Claire E. ; Hales, Klane L. ; Wood, Richard L. ; Mircheff, Austin K. / Stimulation-associated redistribution of Na,K-ATPase in rat lacrimal gland. In: Journal of Membrane Biology. 1988 ; Vol. 102, No. 3. pp. 185-194.
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