Stimulation and partial stabilization of human histidyl-tRNA synthetase by hemoglobin

Tapas Biswas; Frederick W. Miller; Paul H. Plotz

doi:10.1016/0014-5793(88)80827-5

Stimulation and partial stabilization of human histidyl-tRNA synthetase by hemoglobin

Tapas Biswas, Frederick W. Miller, Paul H. Plotz

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Histidyl-tRNA synthetase, an enzyme against which antibodies are directed in some patients with polymyositis, has been purified 5000-fold from HeLa cells, but was extremely labile to dilution or on storage at -80°C. In order to facilitate study of the biochemical and immunological properties of the enzyme, a stabilizer was sought. Hemoglobin at 2 mg/ml was found to stimulate the enzyme and also partially preserved the activity of the enzyme stored at a low concentration (<10μg/ml). Hematin, but not the globin protein, could substitute for hemoglobin in stimulating the enzyme.

Original language	English (US)
Pages (from-to)	203-205
Number of pages	3
Journal	FEBS Letters
Volume	229
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(88)80827-5
State	Published - Feb 29 1988
Externally published	Yes

Keywords

Autoantigen
Hematin
Hemoglobin stabilization
Histidyl-tRNA synthetase

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(88)80827-5

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abstract = "Histidyl-tRNA synthetase, an enzyme against which antibodies are directed in some patients with polymyositis, has been purified 5000-fold from HeLa cells, but was extremely labile to dilution or on storage at -80°C. In order to facilitate study of the biochemical and immunological properties of the enzyme, a stabilizer was sought. Hemoglobin at 2 mg/ml was found to stimulate the enzyme and also partially preserved the activity of the enzyme stored at a low concentration (<10μg/ml). Hematin, but not the globin protein, could substitute for hemoglobin in stimulating the enzyme.",

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AU - Miller, Frederick W.

AU - Plotz, Paul H.

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N2 - Histidyl-tRNA synthetase, an enzyme against which antibodies are directed in some patients with polymyositis, has been purified 5000-fold from HeLa cells, but was extremely labile to dilution or on storage at -80°C. In order to facilitate study of the biochemical and immunological properties of the enzyme, a stabilizer was sought. Hemoglobin at 2 mg/ml was found to stimulate the enzyme and also partially preserved the activity of the enzyme stored at a low concentration (<10μg/ml). Hematin, but not the globin protein, could substitute for hemoglobin in stimulating the enzyme.

AB - Histidyl-tRNA synthetase, an enzyme against which antibodies are directed in some patients with polymyositis, has been purified 5000-fold from HeLa cells, but was extremely labile to dilution or on storage at -80°C. In order to facilitate study of the biochemical and immunological properties of the enzyme, a stabilizer was sought. Hemoglobin at 2 mg/ml was found to stimulate the enzyme and also partially preserved the activity of the enzyme stored at a low concentration (<10μg/ml). Hematin, but not the globin protein, could substitute for hemoglobin in stimulating the enzyme.

KW - Autoantigen

KW - Hematin

KW - Hemoglobin stabilization

KW - Histidyl-tRNA synthetase

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Stimulation and partial stabilization of human histidyl-tRNA synthetase by hemoglobin

Abstract

Keywords

ASJC Scopus subject areas

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