STIM1 fl/fl Ksp-Cre Mouse has Impaired Renal Water Balance

Liudmila Cebotaru, Valeriu Cebotaru, Hua Wang, Lois J. Arend, William B. Guggino

Research output: Contribution to journalArticle

Abstract

Background/AIM: STIM1 is as an essential component in store operated Ca 2+ entry. However give the paucity of information on the role of STIM1 in kidney, the aim was to study the function of STIM1 in the medulla of the kidney. Methods: we crossed a Ksp-cre mouse with another mouse containing two loxP sites flanking Exon 6 of STIM1. The Ksp-cre mouse is based upon the Ksp-cadherin gene promoter which expresses cre recombinase in developing nephrons, collecting ducts (SD) and thick ascending limbs (TAL) of the loop of Henle. Results: The offspring of these mice are viable without gross morphological changes, however, we noticed that the STIM1 Ksp-cre knockout mice produced more urine compared to control. To examine this more carefully, we fed mice low (LP) and high protein (HP) diets respectively. When mice were fed HP diet STIM1 ko mice had significantly increased urinary volume and lower specific gravity compared to wt mice. In STIM1 ko mice fed HP diet urine creatinine and urea were significantly lower compared to wt mice fed HP diet, however the fractional excretion was the same. Conclusion: These data support the idea that STIM1 ko mice have impaired urinary concentrating ability when challenged with HP diet is most likely caused by impaired Ca 2+ -dependent signal transduction through the vasopressin receptor cascade.

Original languageEnglish (US)
Pages (from-to)172-182
Number of pages11
JournalCellular Physiology and Biochemistry
Volume39
Issue number1
DOIs
StatePublished - Jul 1 2016

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Keywords

  • Concentrating Mechanism
  • Kidney
  • STIM1
  • Vasopressin receptor

ASJC Scopus subject areas

  • Physiology

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