STF-IL-4: A novel IL-4-induced signal transducing factor

Chris Schindler; Helena Kashleva; Alessandra Pernis; Richard Pine; Paul Rothman

doi:10.1002/j.1460-2075.1994.tb06388.x

STF-IL-4: A novel IL-4-induced signal transducing factor

Chris Schindler, Helena Kashleva, Alessandra Pernis, Richard Pine, Paul Rothman

Research output: Contribution to journal › Article › peer-review

166 Scopus citations

Abstract

The mechanism by which interleukin-4 (IL-4) regulates the expression of particular genes is unknown. We have determined that IL-4 induces a DNA binding factor (termed STF-IL-4) which has a strong affinity for an IFN-γ activation site (GAS). Interestingly, STF-IL-4 also binds to the IL-4 responsive promoter for the Ig heavy chain germline ε transcript. The IL-4 dependent activation of STF-IL-4 is rapid, does not require protein synthesis and results in the sequential appearance of binding activity first in the cytoplasm and then later in the nucleus. Activation of STF-IL-4 is sensitive to tyrosine kinase inhibitors and the active factor is tyrosine phosphorylated. This pattern of activation is similar to the activation of interferon-induced transcription factors. STF-IL-4 appears to be a new member of a growing family of cytokine-induced transcriptional regulators.

Original language	English (US)
Pages (from-to)	1350-1356
Number of pages	7
Journal	EMBO Journal
Volume	13
Issue number	6
DOIs	https://doi.org/10.1002/j.1460-2075.1994.tb06388.x
State	Published - Mar 15 1994
Externally published	Yes

Keywords

Cytokine
Interferon
Interleukin-4
Signal transduction

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1002/j.1460-2075.1994.tb06388.x

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title = "STF-IL-4: A novel IL-4-induced signal transducing factor",

abstract = "The mechanism by which interleukin-4 (IL-4) regulates the expression of particular genes is unknown. We have determined that IL-4 induces a DNA binding factor (termed STF-IL-4) which has a strong affinity for an IFN-γ activation site (GAS). Interestingly, STF-IL-4 also binds to the IL-4 responsive promoter for the Ig heavy chain germline ε transcript. The IL-4 dependent activation of STF-IL-4 is rapid, does not require protein synthesis and results in the sequential appearance of binding activity first in the cytoplasm and then later in the nucleus. Activation of STF-IL-4 is sensitive to tyrosine kinase inhibitors and the active factor is tyrosine phosphorylated. This pattern of activation is similar to the activation of interferon-induced transcription factors. STF-IL-4 appears to be a new member of a growing family of cytokine-induced transcriptional regulators.",

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doi = "10.1002/j.1460-2075.1994.tb06388.x",

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AU - Schindler, Chris

AU - Kashleva, Helena

AU - Pernis, Alessandra

AU - Pine, Richard

AU - Rothman, Paul

PY - 1994/3/15

Y1 - 1994/3/15

N2 - The mechanism by which interleukin-4 (IL-4) regulates the expression of particular genes is unknown. We have determined that IL-4 induces a DNA binding factor (termed STF-IL-4) which has a strong affinity for an IFN-γ activation site (GAS). Interestingly, STF-IL-4 also binds to the IL-4 responsive promoter for the Ig heavy chain germline ε transcript. The IL-4 dependent activation of STF-IL-4 is rapid, does not require protein synthesis and results in the sequential appearance of binding activity first in the cytoplasm and then later in the nucleus. Activation of STF-IL-4 is sensitive to tyrosine kinase inhibitors and the active factor is tyrosine phosphorylated. This pattern of activation is similar to the activation of interferon-induced transcription factors. STF-IL-4 appears to be a new member of a growing family of cytokine-induced transcriptional regulators.

AB - The mechanism by which interleukin-4 (IL-4) regulates the expression of particular genes is unknown. We have determined that IL-4 induces a DNA binding factor (termed STF-IL-4) which has a strong affinity for an IFN-γ activation site (GAS). Interestingly, STF-IL-4 also binds to the IL-4 responsive promoter for the Ig heavy chain germline ε transcript. The IL-4 dependent activation of STF-IL-4 is rapid, does not require protein synthesis and results in the sequential appearance of binding activity first in the cytoplasm and then later in the nucleus. Activation of STF-IL-4 is sensitive to tyrosine kinase inhibitors and the active factor is tyrosine phosphorylated. This pattern of activation is similar to the activation of interferon-induced transcription factors. STF-IL-4 appears to be a new member of a growing family of cytokine-induced transcriptional regulators.

KW - Cytokine

KW - Interferon

KW - Interleukin-4

KW - Signal transduction

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STF-IL-4: A novel IL-4-induced signal transducing factor

Abstract

Keywords

ASJC Scopus subject areas

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