STF-IL-4: A novel IL-4-induced signal transducing factor

Chris Schindler, Helena Kashleva, Alessandra Pernis, Richard Pine, Paul Rothman

Research output: Contribution to journalArticle

Abstract

The mechanism by which interleukin-4 (IL-4) regulates the expression of particular genes is unknown. We have determined that IL-4 induces a DNA binding factor (termed STF-IL-4) which has a strong affinity for an IFN-γ activation site (GAS). Interestingly, STF-IL-4 also binds to the IL-4 responsive promoter for the Ig heavy chain germline ε transcript. The IL-4 dependent activation of STF-IL-4 is rapid, does not require protein synthesis and results in the sequential appearance of binding activity first in the cytoplasm and then later in the nucleus. Activation of STF-IL-4 is sensitive to tyrosine kinase inhibitors and the active factor is tyrosine phosphorylated. This pattern of activation is similar to the activation of interferon-induced transcription factors. STF-IL-4 appears to be a new member of a growing family of cytokine-induced transcriptional regulators.

Original languageEnglish (US)
Pages (from-to)1350-1356
Number of pages7
JournalEMBO Journal
Volume13
Issue number6
DOIs
StatePublished - Mar 15 1994

Keywords

  • Cytokine
  • Interferon
  • Interleukin-4
  • Signal transduction

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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