Steroid suppression of protein synthesis of activated human lymphocytes

J. C. Rosenberg, K. Lysz, A. Strefling, S. A. Rosenberg

Research output: Contribution to journalArticle

Abstract

In vivo and in vitro studies of the effect of methylprednisolone on protein synthesis of activated lymphocytes has been carried out using [3H]leucine incorporation into lymphocytes as the assay system. PHA and allogeneic cells (two-way MLC) were used to activate the lymphocytes in vitro. It was found that 5 μg/ml of methylprednisolone produced a significant reduction in [3H]leucine incorporation and that higher concentrations did not increase the suppressant effect. Lower concentrations often did produce the same magnitude of suppression. SPS during the MLC was inhibited to a greater extent and at lower concentrations of methylprednisolone than PHA-activated lymphocytes. Patients on maintenance immunosuppression with normally functioning renal allografts had normal SPS responses to PHA. Patients with rejecting renal transplants receiving higher doses of immunosuppressive drugs often had diminished SPS responses to PHA.

Original languageEnglish (US)
Pages (from-to)255-260
Number of pages6
JournalJournal of Surgical Research
Volume20
Issue number3
DOIs
StatePublished - 1976
Externally publishedYes

Fingerprint

Methylprednisolone
Steroids
Lymphocytes
Leucine
Proteins
Kidney
Immunosuppressive Agents
Immunosuppression
Allografts
Maintenance
Transplants
Pharmaceutical Preparations
In Vitro Techniques

ASJC Scopus subject areas

  • Surgery

Cite this

Steroid suppression of protein synthesis of activated human lymphocytes. / Rosenberg, J. C.; Lysz, K.; Strefling, A.; Rosenberg, S. A.

In: Journal of Surgical Research, Vol. 20, No. 3, 1976, p. 255-260.

Research output: Contribution to journalArticle

Rosenberg, J. C. ; Lysz, K. ; Strefling, A. ; Rosenberg, S. A. / Steroid suppression of protein synthesis of activated human lymphocytes. In: Journal of Surgical Research. 1976 ; Vol. 20, No. 3. pp. 255-260.
@article{61843af3f1ce41c59e452b5afa2b1238,
title = "Steroid suppression of protein synthesis of activated human lymphocytes",
abstract = "In vivo and in vitro studies of the effect of methylprednisolone on protein synthesis of activated lymphocytes has been carried out using [3H]leucine incorporation into lymphocytes as the assay system. PHA and allogeneic cells (two-way MLC) were used to activate the lymphocytes in vitro. It was found that 5 μg/ml of methylprednisolone produced a significant reduction in [3H]leucine incorporation and that higher concentrations did not increase the suppressant effect. Lower concentrations often did produce the same magnitude of suppression. SPS during the MLC was inhibited to a greater extent and at lower concentrations of methylprednisolone than PHA-activated lymphocytes. Patients on maintenance immunosuppression with normally functioning renal allografts had normal SPS responses to PHA. Patients with rejecting renal transplants receiving higher doses of immunosuppressive drugs often had diminished SPS responses to PHA.",
author = "Rosenberg, {J. C.} and K. Lysz and A. Strefling and Rosenberg, {S. A.}",
year = "1976",
doi = "10.1016/0022-4804(76)90149-9",
language = "English (US)",
volume = "20",
pages = "255--260",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Steroid suppression of protein synthesis of activated human lymphocytes

AU - Rosenberg, J. C.

AU - Lysz, K.

AU - Strefling, A.

AU - Rosenberg, S. A.

PY - 1976

Y1 - 1976

N2 - In vivo and in vitro studies of the effect of methylprednisolone on protein synthesis of activated lymphocytes has been carried out using [3H]leucine incorporation into lymphocytes as the assay system. PHA and allogeneic cells (two-way MLC) were used to activate the lymphocytes in vitro. It was found that 5 μg/ml of methylprednisolone produced a significant reduction in [3H]leucine incorporation and that higher concentrations did not increase the suppressant effect. Lower concentrations often did produce the same magnitude of suppression. SPS during the MLC was inhibited to a greater extent and at lower concentrations of methylprednisolone than PHA-activated lymphocytes. Patients on maintenance immunosuppression with normally functioning renal allografts had normal SPS responses to PHA. Patients with rejecting renal transplants receiving higher doses of immunosuppressive drugs often had diminished SPS responses to PHA.

AB - In vivo and in vitro studies of the effect of methylprednisolone on protein synthesis of activated lymphocytes has been carried out using [3H]leucine incorporation into lymphocytes as the assay system. PHA and allogeneic cells (two-way MLC) were used to activate the lymphocytes in vitro. It was found that 5 μg/ml of methylprednisolone produced a significant reduction in [3H]leucine incorporation and that higher concentrations did not increase the suppressant effect. Lower concentrations often did produce the same magnitude of suppression. SPS during the MLC was inhibited to a greater extent and at lower concentrations of methylprednisolone than PHA-activated lymphocytes. Patients on maintenance immunosuppression with normally functioning renal allografts had normal SPS responses to PHA. Patients with rejecting renal transplants receiving higher doses of immunosuppressive drugs often had diminished SPS responses to PHA.

UR - http://www.scopus.com/inward/record.url?scp=0017124909&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017124909&partnerID=8YFLogxK

U2 - 10.1016/0022-4804(76)90149-9

DO - 10.1016/0022-4804(76)90149-9

M3 - Article

VL - 20

SP - 255

EP - 260

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 3

ER -