Steroid secretion by the human egg-corona-cumulus complex in culture

Neri Laufer, Alan H. Decherney, Florence P. Haseltine, Harold R. Behrman

Research output: Contribution to journalArticlepeer-review

Abstract

Forty-five oocyte-corona-cumulus complexes (OCCC) were obtained from follicles of 13 women undergoing fertilization in vitro. Follicular growth was induced with human menopausal gonadotropin, and follicular aspiration was performed 36 h after an ovulatory injection of hCG. The maturation of these complexes was evaluated by the extent of cumulus mucification and corona cell dispersal. Three main morphological types were characterized: immature OCCCs (6), with a tight and compact corona-cumulus mass surrounding the oocyte; intermediate OCCCs (26), with a dispersed cumulus but only partlydispersed corona layer; and mature complexes (13) with complete dispersal of both cellular components. During a 24-h culture, progesterone secretion by intermediate and mature OCCCs was 30-fold higher (mean ± SEM, 652 ± 87 ng/OCCC 24 h) than immature OCCCs (19.6 ± 3.5 ng/OCCC 24 h), while estradiol secretion was twice as high (3.8 ± 1.1 vs. 1.2 ± 0.6 ng/OCCC.24 h). Testosterone secretion was similar in all three types of OCCCs cultured (0.30 ng/24 h). It is suggested that the steroids produced by the OCCC may contribute to the local milieu of the taiiopian tube.

Original languageEnglish (US)
Pages (from-to)1153-1157
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume58
Issue number6
DOIs
StatePublished - Jun 1984

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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