TY - JOUR
T1 - Steroid hormones
T2 - modulators of Na+ absorption and Cl- secretion in cultured tracheal epithelia
AU - Zeitlin, Pamela Leslie
AU - Wagner, M.
AU - Markakis, D.
AU - Loughlin, Gerald M.
AU - Guggino, W. B.
PY - 1989
Y1 - 1989
N2 - Cultures of tracheal epithelial cells from adult nonpregnant female rabbits maintained a different balance of Na+ absorption and Cl- secretion from cells of pregnant animals and fetal cultures. Cells of nonpregnant rabbits had a large Na+ absorptive current (11 μA/cm2 or 53% of baseline short circuit current) and a small Cl- secretory current (2.4 μA/cm2 or 11%). In contrast, fetal cells had greater Cl- secretion (15.1 μA/cm2 or 61%) and much less Na+ absorption (1.6 μA/cm2 or <10%). Cells of pregnant animals had amounts of Cl- secretion (11.4 μA/cm2 or 41%) and Na+ absorption (6.7 μA/cm2 or 22%) that were in between that of fetal and adult nonpregnant animals. In addition to these striking differences in baseline ion transport among the three groups, epinephrine was found to stimulate Cl- secretion in cells from pregnant rabbits, both Na+ absorption and Cl- secretion in cells from nonpregnant animals, and neither in fetal cells. Steroid hormones of pregnancy, progesterone and estradiol, when added to cells cultured from nonpregnant rabbits, altered the magnitudes of the individual transport pathways to mimic cells cultured from pregnant animals. In addition to changing baseline transport, these steroids modified the epinephrine-stimulated currents to resemble those in the fetus and in pregnancy. We conclude that steroid hormones regulate the balance of Na+ absorption and Cl-secretion in tracheal epithelia in utero and in adulthood.
AB - Cultures of tracheal epithelial cells from adult nonpregnant female rabbits maintained a different balance of Na+ absorption and Cl- secretion from cells of pregnant animals and fetal cultures. Cells of nonpregnant rabbits had a large Na+ absorptive current (11 μA/cm2 or 53% of baseline short circuit current) and a small Cl- secretory current (2.4 μA/cm2 or 11%). In contrast, fetal cells had greater Cl- secretion (15.1 μA/cm2 or 61%) and much less Na+ absorption (1.6 μA/cm2 or <10%). Cells of pregnant animals had amounts of Cl- secretion (11.4 μA/cm2 or 41%) and Na+ absorption (6.7 μA/cm2 or 22%) that were in between that of fetal and adult nonpregnant animals. In addition to these striking differences in baseline ion transport among the three groups, epinephrine was found to stimulate Cl- secretion in cells from pregnant rabbits, both Na+ absorption and Cl- secretion in cells from nonpregnant animals, and neither in fetal cells. Steroid hormones of pregnancy, progesterone and estradiol, when added to cells cultured from nonpregnant rabbits, altered the magnitudes of the individual transport pathways to mimic cells cultured from pregnant animals. In addition to changing baseline transport, these steroids modified the epinephrine-stimulated currents to resemble those in the fetus and in pregnancy. We conclude that steroid hormones regulate the balance of Na+ absorption and Cl-secretion in tracheal epithelia in utero and in adulthood.
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U2 - 10.1073/pnas.86.7.2502
DO - 10.1073/pnas.86.7.2502
M3 - Article
C2 - 2928347
AN - SCOPUS:0344129235
SN - 0027-8424
VL - 86
SP - 2502
EP - 2505
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 7
ER -