Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex

Antoine M. Valera, Francesca Binda, Sophie A. Pawlowski, Jean Luc Dupont, Jean François Casella, Jeffrey D Rothstein, Bernard Poulain, Philippe Isope

Research output: Contribution to journalArticle

Abstract

Motor coordination is supported by an array of highly organized heterogeneous modules in the cerebellum. How incoming sensorimotor information is channeled and communicated between these anatomical modules is still poorly understood. In this study, we used transgenic mice expressing GFP in specific subsets of Purkinje cells that allowed us to target a given set of cerebellar modules. Combining in vitro recordings and photostimulation, we identified stereotyped patterns of functional synaptic organization between the granule cell layer and its main targets, the Purkinje cells, Golgi cells and molecular layer interneurons. Each type of connection displayed position-specific patterns of granule cell synaptic inputs that do not strictly match with anatomical boundaries but connect distant cortical modules. Although these patterns can be adjusted by activity-dependent processes, they were found to be consistent and predictable between animals. Our results highlight the operational rules underlying communication between modules in the cerebellar cortex.

Original languageEnglish (US)
Article numbere09862
JournaleLife
Volume5
Issue numberMARCH2016
DOIs
StatePublished - Mar 16 2016

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Cerebellar Cortex
Animals
Purkinje Cells
Cells
Communication
Interneurons
Cerebellum
Transgenic Mice

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Valera, A. M., Binda, F., Pawlowski, S. A., Dupont, J. L., Casella, J. F., Rothstein, J. D., ... Isope, P. (2016). Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex. eLife, 5(MARCH2016), [e09862]. https://doi.org/10.7554/eLife.09862

Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex. / Valera, Antoine M.; Binda, Francesca; Pawlowski, Sophie A.; Dupont, Jean Luc; Casella, Jean François; Rothstein, Jeffrey D; Poulain, Bernard; Isope, Philippe.

In: eLife, Vol. 5, No. MARCH2016, e09862, 16.03.2016.

Research output: Contribution to journalArticle

Valera, AM, Binda, F, Pawlowski, SA, Dupont, JL, Casella, JF, Rothstein, JD, Poulain, B & Isope, P 2016, 'Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex', eLife, vol. 5, no. MARCH2016, e09862. https://doi.org/10.7554/eLife.09862
Valera AM, Binda F, Pawlowski SA, Dupont JL, Casella JF, Rothstein JD et al. Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex. eLife. 2016 Mar 16;5(MARCH2016). e09862. https://doi.org/10.7554/eLife.09862
Valera, Antoine M. ; Binda, Francesca ; Pawlowski, Sophie A. ; Dupont, Jean Luc ; Casella, Jean François ; Rothstein, Jeffrey D ; Poulain, Bernard ; Isope, Philippe. / Stereotyped spatial patterns of functional synaptic connectivity in the cerebellar cortex. In: eLife. 2016 ; Vol. 5, No. MARCH2016.
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