Stem-loop IV of Tetrahymena telomerase RNA stimulates processivity in trans

Douglas X. Mason, Elizabeth Goneska, Carol W. Greider

Research output: Contribution to journalArticlepeer-review

Abstract

Telomerase is a ribonucleoprotein enzyme responsible for the addition of telomeres onto the ends of chromosomes. Short or dysfunctional telomeres can lead to cell growth arrest, apoptosis, and genomic instability. Telomerase uses its RNA subunit to copy a short template region for telomere synthesis. To probe for regions of Tetrahymena telomerase RNA essential for function, we assayed 27 circularly permuted RNA deletions for telomerase in vitro activity and binding to the telomerase reverse transcriptase catalytic protein subunit. We found that stem-loop IV is required for wild-type telomerase activity in vitro and will stimulate processivity when added in trans.

Original languageEnglish (US)
Pages (from-to)5606-5613
Number of pages8
JournalMolecular and cellular biology
Volume23
Issue number16
DOIs
StatePublished - Aug 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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