Stem cell profiling by nuclear magnetic resonance spectroscopy

Jacobus F.A. Jansen; Michael J. Shamblott; Peter C.M. Van Zijl; Kimmo K. Lehtimäki; Jeff W.M. Bulte; John D. Gearhart; Juhana M. Hakumäki

doi:10.1002/mrm.20968

Stem cell profiling by nuclear magnetic resonance spectroscopy

Jacobus F.A. Jansen, Michael J. Shamblott, Peter C.M. Van Zijl, Kimmo K. Lehtimäki, Jeff W.M. Bulte, John D. Gearhart, Juhana M. Hakumäki

School of Medicine

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The classification of embryonic and adult stem cells, including their derivatives, is still limited, and often these cells are best defined by their functional properties. Recent gene array studies have yielded contradictory results. Also, very little is known about the metabolic properties of these exciting cells. In this study, proton (¹H) NMR spectroscopy was used to identify the major low-molecular-weight metabolites in murine embryonic stem cells (ESC) and their neural stem cell (NSC) derivatives. ESC are characterized by an unusually low number of NMR-detectable metabolites, high phosphocholine (PC) content, and nondetectable glycerophosphocholine (GPC). The metabolic profiles of NSC resemble glial cells and oligodendrocyte progenitors, but with considerably higher PC, GPC, and myoinositol content. The results suggest that NMR spectroscopy in vitro can provide markers to study the effects of differentiation on cell metabolism, and potentially to assess stem cell preparations for differentiation status.

Original language	English (US)
Pages (from-to)	666-670
Number of pages	5
Journal	Magnetic resonance in medicine
Volume	56
Issue number	3
DOIs	https://doi.org/10.1002/mrm.20968
State	Published - Sep 2006

Keywords

Choline
Metabolism
Myo-inositol
NMR spectroscopy
Stem cells

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

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10.1002/mrm.20968

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title = "Stem cell profiling by nuclear magnetic resonance spectroscopy",

abstract = "The classification of embryonic and adult stem cells, including their derivatives, is still limited, and often these cells are best defined by their functional properties. Recent gene array studies have yielded contradictory results. Also, very little is known about the metabolic properties of these exciting cells. In this study, proton (1H) NMR spectroscopy was used to identify the major low-molecular-weight metabolites in murine embryonic stem cells (ESC) and their neural stem cell (NSC) derivatives. ESC are characterized by an unusually low number of NMR-detectable metabolites, high phosphocholine (PC) content, and nondetectable glycerophosphocholine (GPC). The metabolic profiles of NSC resemble glial cells and oligodendrocyte progenitors, but with considerably higher PC, GPC, and myoinositol content. The results suggest that NMR spectroscopy in vitro can provide markers to study the effects of differentiation on cell metabolism, and potentially to assess stem cell preparations for differentiation status.",

keywords = "Choline, Metabolism, Myo-inositol, NMR spectroscopy, Stem cells",

author = "Jansen, {Jacobus F.A.} and Shamblott, {Michael J.} and {Van Zijl}, {Peter C.M.} and Lehtim{\"a}ki, {Kimmo K.} and Bulte, {Jeff W.M.} and Gearhart, {John D.} and Hakum{\"a}ki, {Juhana M.}",

year = "2006",

month = sep,

doi = "10.1002/mrm.20968",

language = "English (US)",

volume = "56",

pages = "666--670",

journal = "Magnetic resonance in medicine",

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AU - Jansen, Jacobus F.A.

AU - Shamblott, Michael J.

AU - Van Zijl, Peter C.M.

AU - Lehtimäki, Kimmo K.

AU - Bulte, Jeff W.M.

AU - Gearhart, John D.

AU - Hakumäki, Juhana M.

PY - 2006/9

Y1 - 2006/9

N2 - The classification of embryonic and adult stem cells, including their derivatives, is still limited, and often these cells are best defined by their functional properties. Recent gene array studies have yielded contradictory results. Also, very little is known about the metabolic properties of these exciting cells. In this study, proton (1H) NMR spectroscopy was used to identify the major low-molecular-weight metabolites in murine embryonic stem cells (ESC) and their neural stem cell (NSC) derivatives. ESC are characterized by an unusually low number of NMR-detectable metabolites, high phosphocholine (PC) content, and nondetectable glycerophosphocholine (GPC). The metabolic profiles of NSC resemble glial cells and oligodendrocyte progenitors, but with considerably higher PC, GPC, and myoinositol content. The results suggest that NMR spectroscopy in vitro can provide markers to study the effects of differentiation on cell metabolism, and potentially to assess stem cell preparations for differentiation status.

AB - The classification of embryonic and adult stem cells, including their derivatives, is still limited, and often these cells are best defined by their functional properties. Recent gene array studies have yielded contradictory results. Also, very little is known about the metabolic properties of these exciting cells. In this study, proton (1H) NMR spectroscopy was used to identify the major low-molecular-weight metabolites in murine embryonic stem cells (ESC) and their neural stem cell (NSC) derivatives. ESC are characterized by an unusually low number of NMR-detectable metabolites, high phosphocholine (PC) content, and nondetectable glycerophosphocholine (GPC). The metabolic profiles of NSC resemble glial cells and oligodendrocyte progenitors, but with considerably higher PC, GPC, and myoinositol content. The results suggest that NMR spectroscopy in vitro can provide markers to study the effects of differentiation on cell metabolism, and potentially to assess stem cell preparations for differentiation status.

KW - Choline

KW - Metabolism

KW - Myo-inositol

KW - NMR spectroscopy

KW - Stem cells

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JO - Magnetic resonance in medicine

JF - Magnetic resonance in medicine

IS - 3

ER -

Stem cell profiling by nuclear magnetic resonance spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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