Stem cell factor has a suppressive activity to IgE-mediated chemotaxis of mast cells

Junko Sawada, Shinya Shimizu, Takuya Tamatani, Shiro Kanegasaki, Hirohisa Saito, Akane Tanaka, Naotomo Kambe, Tatsutoshi Nakahata, Hiroshi Matsuda

Research output: Contribution to journalArticle

Abstract

Stem cell factor (SCF), which is well known as a cytokine capable of amplifying development and functions of mast cells, is mainly released from fibroblasts in the peripheral tissue. To investigate whether SCF controlled chemotactic migration of mast cells induced by IgE-specific Ag, murine bone marrow-derived cultured mast cells (BMCMC) and human cord blood-derived cultured mast cells (HuCMC) were preincubated with SCF. Although BMCMC and HuCMC sensitized with IgE directly moved toward specific Ag, preincubation for even 1 h with an optimal dose of SCF suppressed the IgE-mediated chemotactic movement. No or little inhibitory effect of SCF was detected in BMCMC derived from c-kit receptor-defect WBB6F1-W/Wv mice. In contrast, preincubation of BMCMC and HuCMC with SCF enhanced β-hexosaminidase release and Ca2+ mobilization in response to Ag after sensitization with IgE. Using the real-time record of chemotactic migration, BMCMC preincubated with SCF manifested motionless without degranulation. These results suggest that locally produced SCF may have an inhibitory effect on chemotaxis of mast cells, contributing to their accumulation and enhancement of functions at the peripheral site in allergic and nonallergic conditions.

Original languageEnglish (US)
Pages (from-to)3626-3632
Number of pages7
JournalJournal of Immunology
Volume174
Issue number6
DOIs
StatePublished - Mar 15 2005
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Sawada, J., Shimizu, S., Tamatani, T., Kanegasaki, S., Saito, H., Tanaka, A., Kambe, N., Nakahata, T., & Matsuda, H. (2005). Stem cell factor has a suppressive activity to IgE-mediated chemotaxis of mast cells. Journal of Immunology, 174(6), 3626-3632. https://doi.org/10.4049/jimmunol.174.6.3626