Stem cell-based therapy for treating limbal stem cells deficiency: A review of different strategies

Research output: Contribution to journalArticle

Abstract

The self renewal capability of limbal epithelial stem (LEST) cells is fundamental to the maintenance and healing of corneal epithelium. Limbal stem cell deficiency (LSCD), due to dysfunction or loss of LEST cells, therefore presents as persistent epithelial defects, corneal vascularization, conjunctivalization etc. Stem cell-based therapy, in its simplest form - limbal autograft, has been used successfully for more than a decade. For bilateral LSCD, similar approaches with limbal allografts have been unsuccessful largely due to strong immune rejection. Therefore, as an alternate strategy for treating bilateral LSCD, ex vivo expansion of the remaining LEST cells or autologous stem cells sourced from other potential sites is being explored. Different culture systems (with and without xenobiotic supplements) using substrates like amniotic membrane or fibrin gels have been used successfully for ex vivo LEST cell maintenance and reproduction by imitating the stem cell niche. This paper is organized into sections reviewing the LEST cells, LSCD and various stem cell-based approaches for treating LSCD and discussing future direction and challenges.

Original languageEnglish (US)
Pages (from-to)188-194
Number of pages7
JournalSaudi Journal of Ophthalmology
Volume28
Issue number3
DOIs
StatePublished - Jul 1 2014

Fingerprint

Cell- and Tissue-Based Therapy
Stem Cells
Epithelial Cells
Maintenance
Stem Cell Niche
Corneal Epithelium
Amnion
Autografts
Xenobiotics
Fibrin
Reproduction
Allografts
Gels

Keywords

  • Limbal autograft
  • Limbal epithelial stem (LEST)
  • Limbal stem cell deficiency (LSCD)

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Stem cell-based therapy for treating limbal stem cells deficiency : A review of different strategies. / He, Hong; Yiu, Samuel Chi-Hung.

In: Saudi Journal of Ophthalmology, Vol. 28, No. 3, 01.07.2014, p. 188-194.

Research output: Contribution to journalArticle

@article{a7adc1e715e849078e123af0bd443a65,
title = "Stem cell-based therapy for treating limbal stem cells deficiency: A review of different strategies",
abstract = "The self renewal capability of limbal epithelial stem (LEST) cells is fundamental to the maintenance and healing of corneal epithelium. Limbal stem cell deficiency (LSCD), due to dysfunction or loss of LEST cells, therefore presents as persistent epithelial defects, corneal vascularization, conjunctivalization etc. Stem cell-based therapy, in its simplest form - limbal autograft, has been used successfully for more than a decade. For bilateral LSCD, similar approaches with limbal allografts have been unsuccessful largely due to strong immune rejection. Therefore, as an alternate strategy for treating bilateral LSCD, ex vivo expansion of the remaining LEST cells or autologous stem cells sourced from other potential sites is being explored. Different culture systems (with and without xenobiotic supplements) using substrates like amniotic membrane or fibrin gels have been used successfully for ex vivo LEST cell maintenance and reproduction by imitating the stem cell niche. This paper is organized into sections reviewing the LEST cells, LSCD and various stem cell-based approaches for treating LSCD and discussing future direction and challenges.",
keywords = "Limbal autograft, Limbal epithelial stem (LEST), Limbal stem cell deficiency (LSCD)",
author = "Hong He and Yiu, {Samuel Chi-Hung}",
year = "2014",
month = "7",
day = "1",
doi = "10.1016/j.sjopt.2014.06.003",
language = "English (US)",
volume = "28",
pages = "188--194",
journal = "Saudi Journal of Ophthalmology",
issn = "1319-4534",
publisher = "King Saud University",
number = "3",

}

TY - JOUR

T1 - Stem cell-based therapy for treating limbal stem cells deficiency

T2 - A review of different strategies

AU - He, Hong

AU - Yiu, Samuel Chi-Hung

PY - 2014/7/1

Y1 - 2014/7/1

N2 - The self renewal capability of limbal epithelial stem (LEST) cells is fundamental to the maintenance and healing of corneal epithelium. Limbal stem cell deficiency (LSCD), due to dysfunction or loss of LEST cells, therefore presents as persistent epithelial defects, corneal vascularization, conjunctivalization etc. Stem cell-based therapy, in its simplest form - limbal autograft, has been used successfully for more than a decade. For bilateral LSCD, similar approaches with limbal allografts have been unsuccessful largely due to strong immune rejection. Therefore, as an alternate strategy for treating bilateral LSCD, ex vivo expansion of the remaining LEST cells or autologous stem cells sourced from other potential sites is being explored. Different culture systems (with and without xenobiotic supplements) using substrates like amniotic membrane or fibrin gels have been used successfully for ex vivo LEST cell maintenance and reproduction by imitating the stem cell niche. This paper is organized into sections reviewing the LEST cells, LSCD and various stem cell-based approaches for treating LSCD and discussing future direction and challenges.

AB - The self renewal capability of limbal epithelial stem (LEST) cells is fundamental to the maintenance and healing of corneal epithelium. Limbal stem cell deficiency (LSCD), due to dysfunction or loss of LEST cells, therefore presents as persistent epithelial defects, corneal vascularization, conjunctivalization etc. Stem cell-based therapy, in its simplest form - limbal autograft, has been used successfully for more than a decade. For bilateral LSCD, similar approaches with limbal allografts have been unsuccessful largely due to strong immune rejection. Therefore, as an alternate strategy for treating bilateral LSCD, ex vivo expansion of the remaining LEST cells or autologous stem cells sourced from other potential sites is being explored. Different culture systems (with and without xenobiotic supplements) using substrates like amniotic membrane or fibrin gels have been used successfully for ex vivo LEST cell maintenance and reproduction by imitating the stem cell niche. This paper is organized into sections reviewing the LEST cells, LSCD and various stem cell-based approaches for treating LSCD and discussing future direction and challenges.

KW - Limbal autograft

KW - Limbal epithelial stem (LEST)

KW - Limbal stem cell deficiency (LSCD)

UR - http://www.scopus.com/inward/record.url?scp=84908022636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908022636&partnerID=8YFLogxK

U2 - 10.1016/j.sjopt.2014.06.003

DO - 10.1016/j.sjopt.2014.06.003

M3 - Article

AN - SCOPUS:84908022636

VL - 28

SP - 188

EP - 194

JO - Saudi Journal of Ophthalmology

JF - Saudi Journal of Ophthalmology

SN - 1319-4534

IS - 3

ER -