@article{8adc646bd1894b0b846505425b3da73c,
title = "Staufen- and FMRP-Containing Neuronal RNPs Are Structurally and Functionally Related to Somatic P Bodies",
abstract = "Local control of mRNA translation modulates neuronal development, synaptic plasticity, and memory formation. A poorly understood aspect of this control is the role and composition of ribonucleoprotein (RNP) particles that mediate transport and translation of neuronal RNAs. Here, we show that staufen- and FMRP-containing RNPs in Drosophila neurons contain proteins also present in somatic {"}P bodies,{"} including the RNA-degradative enzymes Dcp1p and Xrn1p/Pacman and crucial components of miRNA (argonaute), NMD (Upf1p), and general translational repression (Dhh1p/Me31B) pathways. Drosophila Me31B is shown to participate (1) with an FMRP-associated, P body protein (Scd6p/trailer hitch) in FMRP-driven, argonaute-dependent translational repression in developing eye imaginal discs; (2) in dendritic elaboration of larval sensory neurons; and (3) in bantam miRNA-mediated translational repression in wing imaginal discs. These results argue for a conserved mechanism of translational control critical to neuronal function and open up new experimental avenues for understanding the regulation of mRNA function within neurons.",
keywords = "MOLNEURO, RNA, SIGNALING",
author = "Barbee, {Scott A.} and Estes, {Patricia S.} and Cziko, {Anne Marie} and Jens Hillebrand and Luedeman, {Rene A.} and Coller, {Jeff M.} and Nick Johnson and Howlett, {Iris C.} and Cuiyun Geng and Ryu Ueda and Brand, {Andrea H.} and Newbury, {Sarah F.} and Wilhelm, {James E.} and Levine, {Richard B.} and Akira Nakamura and Roy Parker and Mani Ramaswami",
note = "Funding Information: We thank T. Kitamoto, T. Aigaki, W. Grueber, Y.N. Jan, S. Warren, P. Jin, V. Budnik, G. Boulianne, D. St. Johnston, S. Sanyal, J. Brennecke, S. Cohen, the National Institute of Genetics (Japan) fly stock center, and the Bloomington Drosophila stock center for Drosophila stocks; W. Grueber and S. Sanyal for advice; J. Brennecke, E. Holohan, D. Zarnescu, P. Macchi, V. Rodrigues, and M. Kiebler for useful discussions; G. Hannon, T. Jongens, P. Lasko, D. St. Johnston, C. Temme, J. Raff, and the Developmental Studies Hybridoma Bank for antibodies; B. Suter for a Drosophila genomic library; S. Zusman (Genetic Services Inc.) for germline transformation services; C. Boswell of the MCB Imaging Facility for help with microscopy; D. Bentley, P. Jansma, N. Ingraham, and C. Hedgcock of the Arizona Research Labs Divisions of Neurobiology and Biotechnology for access to and instruction in the SEM, Microscopy (tissue sectioning), Tissue Culture Core, and Photo/Graphics Facilities (respectively), as well as their imaging and computing facilities; and G. Bosco for use of the Nikon Eclipse E800 microscope, camera, and software. This work was funded by grants from the NIH/NIDA (DA15495; DA17749) and the Science Foundation of Ireland to M.R., from the Howard Hughes Medical Institute to R.P., from the Ministry of Education, Culture, Sports, Science and Technology, Japan (to A.N.), the UK Biotechnology and Biological Sciences Research Council (S.F.N.), NIH grant GM54409 to Paul MacDonald and an NIH grant to R.B.L. ",
year = "2006",
month = dec,
day = "21",
doi = "10.1016/j.neuron.2006.10.028",
language = "English (US)",
volume = "52",
pages = "997--1009",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",
}