Stationary-phase-inducible ''gearbox'' promoters: Differential effects of katF mutations and role of σ70

D. E. Bohannon, Nancy Connell, J. Keener, A. Tormo, M. Espinosa-Urgel, M. M. Zambrano, R. Kolter

Research output: Contribution to journalArticle

Abstract

Many of the changes in gene expression observed when Escherichia coli cells enter stationary phase are regulated at the level of transcription initiation. A group of stationary-phase-inducible promoters, known as ''gearbox'' promoters, display a characteristic sequence in the -10 region which differs greatly from the consensus sequence for σ70-dependent promoters. Here we describe our studies on the gearbox promoters bolAp1 and mcbAp, responsible for the temporally regulated transcription of bolA and the genes involved in the synthesis of the peptide antibiotic microcin B17, respectively. Deletion analysis of mcbAp demonstrated that the stationary-phase-inducible properties of this promoter are found in a DNA fragment extending from -54 to +11 bp, surrounding the transcriptional start site, and are separable from DNA sequences responsible for the OmpR-dependent stimulation of transcription of mcbAp. In vitro transcription studies indicate that the RNA polymerase holoenzyme involved in the transcription of mcbAp contains σ70. In this and an accompanying paper (R. Lange and R. Hengge-Aronis, J. Bacteriol. 173:4474-4481, 1991), experiments are described which show that the product of katF, a global regulator of stationary-phase gene expression and a putative sigma factor, is required for the expression of bolAp1 fused to the reporter gene lacZ. In contrast, mcbAp appears to be negatively regulated by katF. We discuss the implications of these results for postexponential gene expression and the role of gearbox sequences in the regulation of promoter activity.

Original languageEnglish (US)
Pages (from-to)4482-4492
Number of pages11
JournalJournal of Bacteriology
Volume173
Issue number14
StatePublished - Aug 30 1991
Externally publishedYes

Fingerprint

Gene Expression
Mutation
Sigma Factor
Holoenzymes
Consensus Sequence
DNA-Directed RNA Polymerases
Reporter Genes
Escherichia coli
Anti-Bacterial Agents
Peptides
DNA
Genes
microcin
In Vitro Techniques

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Bohannon, D. E., Connell, N., Keener, J., Tormo, A., Espinosa-Urgel, M., Zambrano, M. M., & Kolter, R. (1991). Stationary-phase-inducible ''gearbox'' promoters: Differential effects of katF mutations and role of σ70. Journal of Bacteriology, 173(14), 4482-4492.

Stationary-phase-inducible ''gearbox'' promoters : Differential effects of katF mutations and role of σ70. / Bohannon, D. E.; Connell, Nancy; Keener, J.; Tormo, A.; Espinosa-Urgel, M.; Zambrano, M. M.; Kolter, R.

In: Journal of Bacteriology, Vol. 173, No. 14, 30.08.1991, p. 4482-4492.

Research output: Contribution to journalArticle

Bohannon, DE, Connell, N, Keener, J, Tormo, A, Espinosa-Urgel, M, Zambrano, MM & Kolter, R 1991, 'Stationary-phase-inducible ''gearbox'' promoters: Differential effects of katF mutations and role of σ70', Journal of Bacteriology, vol. 173, no. 14, pp. 4482-4492.
Bohannon DE, Connell N, Keener J, Tormo A, Espinosa-Urgel M, Zambrano MM et al. Stationary-phase-inducible ''gearbox'' promoters: Differential effects of katF mutations and role of σ70. Journal of Bacteriology. 1991 Aug 30;173(14):4482-4492.
Bohannon, D. E. ; Connell, Nancy ; Keener, J. ; Tormo, A. ; Espinosa-Urgel, M. ; Zambrano, M. M. ; Kolter, R. / Stationary-phase-inducible ''gearbox'' promoters : Differential effects of katF mutations and role of σ70. In: Journal of Bacteriology. 1991 ; Vol. 173, No. 14. pp. 4482-4492.
@article{0562df9e0ee2401baea3940dd243488b,
title = "Stationary-phase-inducible ''gearbox'' promoters: Differential effects of katF mutations and role of σ70",
abstract = "Many of the changes in gene expression observed when Escherichia coli cells enter stationary phase are regulated at the level of transcription initiation. A group of stationary-phase-inducible promoters, known as ''gearbox'' promoters, display a characteristic sequence in the -10 region which differs greatly from the consensus sequence for σ70-dependent promoters. Here we describe our studies on the gearbox promoters bolAp1 and mcbAp, responsible for the temporally regulated transcription of bolA and the genes involved in the synthesis of the peptide antibiotic microcin B17, respectively. Deletion analysis of mcbAp demonstrated that the stationary-phase-inducible properties of this promoter are found in a DNA fragment extending from -54 to +11 bp, surrounding the transcriptional start site, and are separable from DNA sequences responsible for the OmpR-dependent stimulation of transcription of mcbAp. In vitro transcription studies indicate that the RNA polymerase holoenzyme involved in the transcription of mcbAp contains σ70. In this and an accompanying paper (R. Lange and R. Hengge-Aronis, J. Bacteriol. 173:4474-4481, 1991), experiments are described which show that the product of katF, a global regulator of stationary-phase gene expression and a putative sigma factor, is required for the expression of bolAp1 fused to the reporter gene lacZ. In contrast, mcbAp appears to be negatively regulated by katF. We discuss the implications of these results for postexponential gene expression and the role of gearbox sequences in the regulation of promoter activity.",
author = "Bohannon, {D. E.} and Nancy Connell and J. Keener and A. Tormo and M. Espinosa-Urgel and Zambrano, {M. M.} and R. Kolter",
year = "1991",
month = "8",
day = "30",
language = "English (US)",
volume = "173",
pages = "4482--4492",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "14",

}

TY - JOUR

T1 - Stationary-phase-inducible ''gearbox'' promoters

T2 - Differential effects of katF mutations and role of σ70

AU - Bohannon, D. E.

AU - Connell, Nancy

AU - Keener, J.

AU - Tormo, A.

AU - Espinosa-Urgel, M.

AU - Zambrano, M. M.

AU - Kolter, R.

PY - 1991/8/30

Y1 - 1991/8/30

N2 - Many of the changes in gene expression observed when Escherichia coli cells enter stationary phase are regulated at the level of transcription initiation. A group of stationary-phase-inducible promoters, known as ''gearbox'' promoters, display a characteristic sequence in the -10 region which differs greatly from the consensus sequence for σ70-dependent promoters. Here we describe our studies on the gearbox promoters bolAp1 and mcbAp, responsible for the temporally regulated transcription of bolA and the genes involved in the synthesis of the peptide antibiotic microcin B17, respectively. Deletion analysis of mcbAp demonstrated that the stationary-phase-inducible properties of this promoter are found in a DNA fragment extending from -54 to +11 bp, surrounding the transcriptional start site, and are separable from DNA sequences responsible for the OmpR-dependent stimulation of transcription of mcbAp. In vitro transcription studies indicate that the RNA polymerase holoenzyme involved in the transcription of mcbAp contains σ70. In this and an accompanying paper (R. Lange and R. Hengge-Aronis, J. Bacteriol. 173:4474-4481, 1991), experiments are described which show that the product of katF, a global regulator of stationary-phase gene expression and a putative sigma factor, is required for the expression of bolAp1 fused to the reporter gene lacZ. In contrast, mcbAp appears to be negatively regulated by katF. We discuss the implications of these results for postexponential gene expression and the role of gearbox sequences in the regulation of promoter activity.

AB - Many of the changes in gene expression observed when Escherichia coli cells enter stationary phase are regulated at the level of transcription initiation. A group of stationary-phase-inducible promoters, known as ''gearbox'' promoters, display a characteristic sequence in the -10 region which differs greatly from the consensus sequence for σ70-dependent promoters. Here we describe our studies on the gearbox promoters bolAp1 and mcbAp, responsible for the temporally regulated transcription of bolA and the genes involved in the synthesis of the peptide antibiotic microcin B17, respectively. Deletion analysis of mcbAp demonstrated that the stationary-phase-inducible properties of this promoter are found in a DNA fragment extending from -54 to +11 bp, surrounding the transcriptional start site, and are separable from DNA sequences responsible for the OmpR-dependent stimulation of transcription of mcbAp. In vitro transcription studies indicate that the RNA polymerase holoenzyme involved in the transcription of mcbAp contains σ70. In this and an accompanying paper (R. Lange and R. Hengge-Aronis, J. Bacteriol. 173:4474-4481, 1991), experiments are described which show that the product of katF, a global regulator of stationary-phase gene expression and a putative sigma factor, is required for the expression of bolAp1 fused to the reporter gene lacZ. In contrast, mcbAp appears to be negatively regulated by katF. We discuss the implications of these results for postexponential gene expression and the role of gearbox sequences in the regulation of promoter activity.

UR - http://www.scopus.com/inward/record.url?scp=0025772545&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025772545&partnerID=8YFLogxK

M3 - Article

C2 - 1906064

AN - SCOPUS:0025772545

VL - 173

SP - 4482

EP - 4492

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 14

ER -