Staphylococcal protein A-enzyme immunoglobulin conjugates: Versatile tools for enzyme immunoassays

Robert H. Yolken, Flora J. Leister

Research output: Contribution to journalArticle

Abstract

This report described the use of peroxidase-labeled staphylococcal protein A to prepare conjugates suitable for direct enzyme immunoassays (EIAs). Such conjugates were used to develop direct EIA systems for the measurement of two antigens associated with human infections, human rotavirus and Haemophilus influenzae type b polysaccharide. Both systems were as sensitive or more sensitive than currently available EIAs for the measurement of standard antigens. In addition, both systems could be utilized to correctly identify the antigens in clinical specimens obtained from sick patients. Efficient enzyme conjugates prepared with enzyme-labeled staphylococcal protein A were simple to formulate providing that immunoglobulin of the appropriate animal species was available as the source of antibody. The use of such conjugates might increase the availability of practical EIA systems for the measurement of a wide range of medically important antigens.

Original languageEnglish (US)
Pages (from-to)209-218
Number of pages10
JournalJournal of Immunological Methods
Volume43
Issue number2
DOIs
StatePublished - Jun 16 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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