TY - JOUR
T1 - Staphylococcal protein A-enzyme immunoglobulin conjugates
T2 - Versatile tools for enzyme immunoassays
AU - Yolken, Robert H.
AU - Leister, Flora J.
N1 - Funding Information:
1 Supported by The Hospital for Consumptives of Maryland (Eudowood), Baltimore, MD, Contract No. NO1 AI 92616, N.I.A.I.D., National Institutes of Health, Bethesda, MD, and The Thrasher Research Fund, Salt Lake City, UT, U.S.A.
PY - 1981/6/16
Y1 - 1981/6/16
N2 - This report described the use of peroxidase-labeled staphylococcal protein A to prepare conjugates suitable for direct enzyme immunoassays (EIAs). Such conjugates were used to develop direct EIA systems for the measurement of two antigens associated with human infections, human rotavirus and Haemophilus influenzae type b polysaccharide. Both systems were as sensitive or more sensitive than currently available EIAs for the measurement of standard antigens. In addition, both systems could be utilized to correctly identify the antigens in clinical specimens obtained from sick patients. Efficient enzyme conjugates prepared with enzyme-labeled staphylococcal protein A were simple to formulate providing that immunoglobulin of the appropriate animal species was available as the source of antibody. The use of such conjugates might increase the availability of practical EIA systems for the measurement of a wide range of medically important antigens.
AB - This report described the use of peroxidase-labeled staphylococcal protein A to prepare conjugates suitable for direct enzyme immunoassays (EIAs). Such conjugates were used to develop direct EIA systems for the measurement of two antigens associated with human infections, human rotavirus and Haemophilus influenzae type b polysaccharide. Both systems were as sensitive or more sensitive than currently available EIAs for the measurement of standard antigens. In addition, both systems could be utilized to correctly identify the antigens in clinical specimens obtained from sick patients. Efficient enzyme conjugates prepared with enzyme-labeled staphylococcal protein A were simple to formulate providing that immunoglobulin of the appropriate animal species was available as the source of antibody. The use of such conjugates might increase the availability of practical EIA systems for the measurement of a wide range of medically important antigens.
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U2 - 10.1016/0022-1759(81)90025-9
DO - 10.1016/0022-1759(81)90025-9
M3 - Article
C2 - 6267138
AN - SCOPUS:0019460213
VL - 43
SP - 209
EP - 218
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 2
ER -