TY - JOUR
T1 - Staphylococcal peptidoglycan initiates an inflammatory response and procoagulant activity in human vascular endothelial cells
T2 - A comparison with highly purified lipoteichoic acid and TSST-1
AU - Mattsson, Eva
AU - Heying, Ruth
AU - Van De Gevel, Joke S.
AU - Hartung, Thomas
AU - Beekhuizen, Henry
PY - 2008/1
Y1 - 2008/1
N2 - Staphylocoocus aureus is one of the most significant pathogens in human sepsis and endocarditis. A hallmark of these endovascular S. aureus infections is that the coagulation system is triggered by a tissue factor (TF)-dependent pathway. This study demonstrates that highly purified S. aureus peptidoglycan, lipoteichoic acid (LTA) and TSST-1 increase TF mRNA and TF surface protein in human umbilical vein endothelial cells (ECs). Concomitantly, peptidoglycan- and LTA-activated ECs express significant TF-dependent procoagulant activity (TF PCA). In addition peptidoglycan, but not LTA or TSST-1, induced surface expression of the EC inflammation markers ICAM-1 and VCAM-1, which supported the adhesion of monocytes to these ECs. During the coculture of peptidoglycan-activated ECs and adherent monocytes, a marked additional increase of TF PCA was observed. Marginal increases in TF PCA were observed in cocultures of monocytes with LTA- or TSST-1-activated ECs. This study defines in particular staphylococcal peptidoglycan, previously known as a potent initiator of TF PCA in monocytes, as also being an activator of a coagulant response in human ECs that is further intensified by the presence of surface-bound monocytes.
AB - Staphylocoocus aureus is one of the most significant pathogens in human sepsis and endocarditis. A hallmark of these endovascular S. aureus infections is that the coagulation system is triggered by a tissue factor (TF)-dependent pathway. This study demonstrates that highly purified S. aureus peptidoglycan, lipoteichoic acid (LTA) and TSST-1 increase TF mRNA and TF surface protein in human umbilical vein endothelial cells (ECs). Concomitantly, peptidoglycan- and LTA-activated ECs express significant TF-dependent procoagulant activity (TF PCA). In addition peptidoglycan, but not LTA or TSST-1, induced surface expression of the EC inflammation markers ICAM-1 and VCAM-1, which supported the adhesion of monocytes to these ECs. During the coculture of peptidoglycan-activated ECs and adherent monocytes, a marked additional increase of TF PCA was observed. Marginal increases in TF PCA were observed in cocultures of monocytes with LTA- or TSST-1-activated ECs. This study defines in particular staphylococcal peptidoglycan, previously known as a potent initiator of TF PCA in monocytes, as also being an activator of a coagulant response in human ECs that is further intensified by the presence of surface-bound monocytes.
KW - Coagulation
KW - Endothelial cell
KW - Lipoteichoic acid
KW - Peptidoglycan
KW - TSST-1
KW - Tissue factor
UR - http://www.scopus.com/inward/record.url?scp=38149071787&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38149071787&partnerID=8YFLogxK
U2 - 10.1111/j.1574-695X.2007.00350.x
DO - 10.1111/j.1574-695X.2007.00350.x
M3 - Article
C2 - 18031538
AN - SCOPUS:38149071787
SN - 0928-8244
VL - 52
SP - 110
EP - 117
JO - FEMS Immunology and Medical Microbiology
JF - FEMS Immunology and Medical Microbiology
IS - 1
ER -