Staphylococcal peptidoglycan initiates an inflammatory response and procoagulant activity in human vascular endothelial cells

A comparison with highly purified lipoteichoic acid and TSST-1

Eva Mattsson, Ruth Heying, Joke S. Van De Gevel, Thomas Hartung, Henry Beekhuizen

Research output: Contribution to journalArticle

Abstract

Staphylocoocus aureus is one of the most significant pathogens in human sepsis and endocarditis. A hallmark of these endovascular S. aureus infections is that the coagulation system is triggered by a tissue factor (TF)-dependent pathway. This study demonstrates that highly purified S. aureus peptidoglycan, lipoteichoic acid (LTA) and TSST-1 increase TF mRNA and TF surface protein in human umbilical vein endothelial cells (ECs). Concomitantly, peptidoglycan- and LTA-activated ECs express significant TF-dependent procoagulant activity (TF PCA). In addition peptidoglycan, but not LTA or TSST-1, induced surface expression of the EC inflammation markers ICAM-1 and VCAM-1, which supported the adhesion of monocytes to these ECs. During the coculture of peptidoglycan-activated ECs and adherent monocytes, a marked additional increase of TF PCA was observed. Marginal increases in TF PCA were observed in cocultures of monocytes with LTA- or TSST-1-activated ECs. This study defines in particular staphylococcal peptidoglycan, previously known as a potent initiator of TF PCA in monocytes, as also being an activator of a coagulant response in human ECs that is further intensified by the presence of surface-bound monocytes.

Original languageEnglish (US)
Pages (from-to)110-117
Number of pages8
JournalFEMS Immunology and Medical Microbiology
Volume52
Issue number1
DOIs
StatePublished - Jan 2008
Externally publishedYes

Fingerprint

Peptidoglycan
Thromboplastin
Human Activities
Endothelial Cells
Monocytes
Passive Cutaneous Anaphylaxis
Coculture Techniques
Coagulants
Vascular Cell Adhesion Molecule-1
Human Umbilical Vein Endothelial Cells
Intercellular Adhesion Molecule-1
Endocarditis
Staphylococcal enterotoxin F
lipoteichoic acid
Sepsis
Membrane Proteins
Inflammation
Messenger RNA
Infection

Keywords

  • Coagulation
  • Endothelial cell
  • Lipoteichoic acid
  • Peptidoglycan
  • Tissue factor
  • TSST-1

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

Staphylococcal peptidoglycan initiates an inflammatory response and procoagulant activity in human vascular endothelial cells : A comparison with highly purified lipoteichoic acid and TSST-1. / Mattsson, Eva; Heying, Ruth; Van De Gevel, Joke S.; Hartung, Thomas; Beekhuizen, Henry.

In: FEMS Immunology and Medical Microbiology, Vol. 52, No. 1, 01.2008, p. 110-117.

Research output: Contribution to journalArticle

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