Standardized susceptibility testing of fluconazole: An international collaborative study

M. A. Pfaller, B. Dupont, G. S. Kobayashi, J. Muller, M. G. Rinaldi, A. Espinel- Ingroff, S. Shadomy, P. F. Troke, T. J. Walsh, D. W. Warnock

Research output: Contribution to journalArticle

Abstract

An international collaborative study of broth dilution (MIC) and disk diffusion susceptibility testing of fluconazole was conducted by using a chemically defined medium (High-Resolution Antifungal Assay Medium; Oxoid Ltd., Basingstoke, United Kingdom) and standard test methods performed in eight reference laboratories. Ten yeast isolates were tested by each test method in duplicate on each of 3 separate days. The intralaboratory reproducibility of the MIC test was excellent; 95.7% of the replicate tests (n = 220) were within 2 doubling dilutions of the other values in the set for the eight laboratories. The intralaboratory reproducibility of the disk test was also good, with 91% of the replicate tests (n = 234) agreeing with each other within an arbitrarily chosen value of 4 mm. Interlaboratory agreement of MIC test results was acceptable, with 84% of the MICs agreeing within 2 doubling dilutions. In contrast, the interlaboratory agreement of the disk test was not good, with only 59% of test results agreeing within 4 mm. Comparison of the rank order of MICs obtained in each laboratory with the reference rank order gave an agreement of 70 to 80% (median, 80%) with the MIC test and 70 to 90% (median, 80%) with the disk test. These preliminary results are encouraging for the development of standardized testing methods for testing fluconazole.

Original languageEnglish (US)
Pages (from-to)1805-1809
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume36
Issue number9
StatePublished - 1992
Externally publishedYes

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Fluconazole
Yeasts

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Pfaller, M. A., Dupont, B., Kobayashi, G. S., Muller, J., Rinaldi, M. G., Espinel- Ingroff, A., ... Warnock, D. W. (1992). Standardized susceptibility testing of fluconazole: An international collaborative study. Antimicrobial Agents and Chemotherapy, 36(9), 1805-1809.

Standardized susceptibility testing of fluconazole : An international collaborative study. / Pfaller, M. A.; Dupont, B.; Kobayashi, G. S.; Muller, J.; Rinaldi, M. G.; Espinel- Ingroff, A.; Shadomy, S.; Troke, P. F.; Walsh, T. J.; Warnock, D. W.

In: Antimicrobial Agents and Chemotherapy, Vol. 36, No. 9, 1992, p. 1805-1809.

Research output: Contribution to journalArticle

Pfaller, MA, Dupont, B, Kobayashi, GS, Muller, J, Rinaldi, MG, Espinel- Ingroff, A, Shadomy, S, Troke, PF, Walsh, TJ & Warnock, DW 1992, 'Standardized susceptibility testing of fluconazole: An international collaborative study', Antimicrobial Agents and Chemotherapy, vol. 36, no. 9, pp. 1805-1809.
Pfaller MA, Dupont B, Kobayashi GS, Muller J, Rinaldi MG, Espinel- Ingroff A et al. Standardized susceptibility testing of fluconazole: An international collaborative study. Antimicrobial Agents and Chemotherapy. 1992;36(9):1805-1809.
Pfaller, M. A. ; Dupont, B. ; Kobayashi, G. S. ; Muller, J. ; Rinaldi, M. G. ; Espinel- Ingroff, A. ; Shadomy, S. ; Troke, P. F. ; Walsh, T. J. ; Warnock, D. W. / Standardized susceptibility testing of fluconazole : An international collaborative study. In: Antimicrobial Agents and Chemotherapy. 1992 ; Vol. 36, No. 9. pp. 1805-1809.
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