Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp)

Justin Bailey, Richard A. Urbanowicz, Jonathan K. Ball, Mansun Law, Steven K.H. Foung

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface are released through a retroviral budding process into the supernatant. Viral E1E2 glycoproteins facilitate a single round of receptor-mediated entry of HCVpp into hepatoma cells, which can be quantified by reporter gene expression. These HCVpp have been employed to study mechanisms of HCV entry into hepatoma cells, as well as HCV neutralization by immune sera or HCV-specific monoclonal antibodies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages441-450
Number of pages10
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1911
ISSN (Print)1064-3745

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Keywords

  • Flaviviridae
  • HCV pseudoparticles
  • Hepatitis C virus
  • Neutralizing antibodies
  • Neutralizing breadth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bailey, J., Urbanowicz, R. A., Ball, J. K., Law, M., & Foung, S. K. H. (2019). Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp). In Methods in Molecular Biology (pp. 441-450). (Methods in Molecular Biology; Vol. 1911). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8976-8_30