Standardization of laboratory methods for the PERCH study

Amanda J. Driscoll, Ruth A. Karron, Susan C. Morpeth, Niranjan Bhat, Orin S. Levine, Henry C. Baggett, W. Abdullah Brooks, Daniel R. Feikin, Laura L. Hammitt, Stephen R.C. Howie, Maria Deloria Knoll, Karen L. Kotloff, Shabir A. Madhi, J. Anthony G. Scott, Donald M. Thea, Peter V. Adrian, Dilruba Ahmed, Muntasir Alam, Trevor P. Anderson, Martin AntonioVicky L. Baillie, Michel Dione, Hubert P. Endtz, Caroline Gitahi, Angela Karani, Geoffrey Kwenda, Abdoul Aziz Maiga, Jessica McClellan, Joanne L. Mitchell, Palesa Morailane, Daisy Mugo, John Mwaba, James Mwansa, Salim Mwarumba, Sammy Nyongesa, Sandra Panchalingam, Mustafizur Rahman, Pongpun Sawatwong, Boubou Tamboura, Aliou Toure, Toni Whistler, Katherine L. O'Brien, David R. Murdoch

Research output: Contribution to journalArticlepeer-review

Abstract

The Pneumonia Etiology Research for Child Health study was conducted across diverse research sites and relied on standardized clinical and laboratory methods for the accurate and meaningful interpretation of pneumonia etiology data. Blood, respiratory specimens, and urine were collected from children aged 1-5months hospitalized with severe or very severe pneumonia and community controls of the same age without severe pneumonia and were tested with an extensive array of laboratory diagnostic tests. A standardized testing algorithm and standard operating procedures were applied across all study sites. Site laboratories received uniform training, equipment, and reagents for core testing methods. Standardization was further assured by routine teleconferences, in-person meetings, site monitoring visits, and internal and external quality assurance testing. Targeted confirmatory testing and testing by specialized assays were done at a central reference laboratory.

Original languageEnglish (US)
Pages (from-to)S245-S252
JournalClinical Infectious Diseases
Volume64
DOIs
StatePublished - 2017

Keywords

  • Laboratory
  • PERCH
  • Pneumonia
  • Respiratory infection

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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