Stage-dependent olig2 expression in motor neurons and oligodendrocytes differentiated from embryonic stem cells

Soojung Shin, Haipeng Xue, Mark P. Mattson, Mahendra S. Rao

Research output: Contribution to journalArticle

Abstract

Although embryonic stem (ES) cells are capable of forming any cell type in the body, the mechanisms that control cell type-specific differentiation are largely unknown. In the present study, we examined the process of differentiation to motor neurons and oligodendrocytes from mouse (Olig2-GFP) ES cells. Mouse ES cells undergo a sequential process of differentiation over a 3-week period to generate motor neurons and oligodendrocytes. At day 7 of differentiation, Olig2-expressing cells are biased to a neuronal lineage. However, further differentiation (day 32) resulted in the majority of Olig2-expressing cells exhibiting an oligodendrocyte phenotype as well as a reduced ability to make motor neurons. Exposure of human ES cells to Sonic hedgehog (Shh) likewise resulted in enhanced motor neuron differentiation. Our results establish the requirements for directing ES cells to become motor neurons and oligodendrocytes and show that ES cell-derived Olig2+ cells can give rise to both motor neurons and oligodendrocytes, depending on the time at which differentiation is initiated.

Original languageEnglish (US)
Pages (from-to)131-141
Number of pages11
JournalStem Cells and Development
Volume16
Issue number1
DOIs
StatePublished - Feb 2007
Externally publishedYes

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Oligodendroglia
Motor Neurons
Embryonic Stem Cells
Hedgehogs
Cell Differentiation
Phenotype

ASJC Scopus subject areas

  • Hematology

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Stage-dependent olig2 expression in motor neurons and oligodendrocytes differentiated from embryonic stem cells. / Shin, Soojung; Xue, Haipeng; Mattson, Mark P.; Rao, Mahendra S.

In: Stem Cells and Development, Vol. 16, No. 1, 02.2007, p. 131-141.

Research output: Contribution to journalArticle

Shin, Soojung ; Xue, Haipeng ; Mattson, Mark P. ; Rao, Mahendra S. / Stage-dependent olig2 expression in motor neurons and oligodendrocytes differentiated from embryonic stem cells. In: Stem Cells and Development. 2007 ; Vol. 16, No. 1. pp. 131-141.
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