Stable intronic sequence RNA (sisRNA), a new class of noncoding RNA from the oocyte nucleus of Xenopus tropicalis

Eugene J. Gardner, Zehra F. Nizami, Jr Conover Talbot, Joseph G. Gall

Research output: Contribution to journalArticle

Abstract

To compare nuclear and cytoplasmic RNA from a single cell type, free of cross-contamination, we studied the oocyte of the frog Xenopus tropicalis, a giant cell with an equally giant nucleus. We isolated RNA from manually dissected nuclei and cytoplasm of mature oocytes and subjected it to deep sequencing. Cytoplasmic mRNA consisted primarily of spliced exons derived from ~6700 annotated genes. Nearly all of these genes were represented in the nucleus by intronic sequences. However, unspliced nascent transcripts were not detected. Inhibition of transcription or splicing for 1-2 d had little or no effect on the abundance of nuclear intronic sequences, demonstrating that they are unusually stable. RT-PCR analysis showed that these stable intronic sequences are transcribed from the coding strand and that a given intron can be processed into more than one molecule. Stable intronic sequence RNA (sisRNA) from the oocyte nucleus constitutes a new class of noncoding RNA. sisRNA is detectable by RT-PCR in samples of total RNA from embryos up to the mid-blastula stage, when zygotic transcription begins. Storage of sisRNA in the oocyte nucleus and its transmission to the developing embryo suggest that it may play important regulatory roles during oogenesis and/or early embryogenesis.

Original languageEnglish (US)
Pages (from-to)2550-2559
Number of pages10
JournalGenes & development
Volume26
Issue number22
DOIs
StatePublished - Nov 15 2012

Fingerprint

Untranslated RNA
Xenopus
Oocytes
Embryonic Structures
RNA
Blastula
Nuclear RNA
High-Throughput Nucleotide Sequencing
Polymerase Chain Reaction
Oogenesis
Giant Cells
Anura
Introns
Genes
Embryonic Development
Exons
Cytoplasm
Messenger RNA

Keywords

  • Intron
  • Lampbrush chromosome
  • Noncoding RNA
  • Oocyte
  • Transcription
  • Xenopus

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Stable intronic sequence RNA (sisRNA), a new class of noncoding RNA from the oocyte nucleus of Xenopus tropicalis. / Gardner, Eugene J.; Nizami, Zehra F.; Conover Talbot, Jr; Gall, Joseph G.

In: Genes & development, Vol. 26, No. 22, 15.11.2012, p. 2550-2559.

Research output: Contribution to journalArticle

Gardner, Eugene J. ; Nizami, Zehra F. ; Conover Talbot, Jr ; Gall, Joseph G. / Stable intronic sequence RNA (sisRNA), a new class of noncoding RNA from the oocyte nucleus of Xenopus tropicalis. In: Genes & development. 2012 ; Vol. 26, No. 22. pp. 2550-2559.
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