Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination

Josiane Eid, Barbara Sollner-Webb

Research output: Contribution to journalArticle

Abstract

A calmodulin-neomycin-resistance fusion gene was introduced into Trypanosoma brucei by electroporation, and stably transformed cell lines were obtained. In all of the transformants, the fusion gene had integrated into the host genome at the cognate locus, evidently by homologous recombination within flanking calmodulin DNA. This unusual observation distinguishes trypanosomes as the only eukaryote other than yeast known to undergo gene targeting in essentially 100% of the stable transformants. It should now be possible to systematically manipulate the trypanosome genome, directing predetermined mutations to virtually any chromosomal locus.

Original languageEnglish (US)
Pages (from-to)2118-2121
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number6
StatePublished - 1991

Fingerprint

Trypanosoma brucei brucei
Trypanosomiasis
Gene Fusion
Homologous Recombination
Calmodulin
Genome
Transformed Cell Line
Electroporation
Neomycin
Gene Targeting
Eukaryota
Yeasts
Observation
Mutation
DNA

Keywords

  • Calmodulin gene
  • Gene targeting
  • Homologous integration
  • Neomvein-resistance gene
  • Transfection

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination. / Eid, Josiane; Sollner-Webb, Barbara.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 6, 1991, p. 2118-2121.

Research output: Contribution to journalArticle

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