Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination

Josiane Eid, Barbara Sollner-Webb

Research output: Contribution to journalArticlepeer-review

Abstract

A calmodulin-neomycin-resistance fusion gene was introduced into Trypanosoma brucei by electroporation, and stably transformed cell lines were obtained. In all of the transformants, the fusion gene had integrated into the host genome at the cognate locus, evidently by homologous recombination within flanking calmodulin DNA. This unusual observation distinguishes trypanosomes as the only eukaryote other than yeast known to undergo gene targeting in essentially 100% of the stable transformants. It should now be possible to systematically manipulate the trypanosome genome, directing predetermined mutations to virtually any chromosomal locus.

Original languageEnglish (US)
Pages (from-to)2118-2121
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number6
DOIs
StatePublished - 1991

Keywords

  • Calmodulin gene
  • Gene targeting
  • Homologous integration
  • Neomvein-resistance gene
  • Transfection

ASJC Scopus subject areas

  • General

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