Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response

Stephany Tzeng, Kevin J. McHugh, Adam M. Behrens, Sviatlana Rose, James L. Sugarman, Shiran Ferber, Robert Langer, Ana Jaklenec

Research output: Contribution to journalArticle

Abstract

Vaccination in the developing world is hampered by limited patient access, which prevents individuals from receiving the multiple injections necessary for protective immunity. Here, we developed an injectable microparticle formulation of the inactivated polio vaccine (IPV) that releases multiple pulses of stable antigen over time. To accomplish this, we established an IPV stabilization strategy using cationic polymers for pH modulation to enhance traditional small-molecule–based stabilization methods. We investigated the mechanism of this strategy and showed that it was broadly applicable to all three antigens in IPV. Our lead formulations released two bursts of IPV 1 month apart, mimicking a typical vaccination schedule in the developing world. One injection of the controlled-release formulations elicited a similar or better neutralizing response in rats, considered the correlate of protection in humans, than multiple injections of liquid vaccine. This single-administration vaccine strategy has the potential to improve vaccine coverage in the developing world.

Original languageEnglish (US)
Pages (from-to)E5269-E5278
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number23
DOIs
StatePublished - Jun 5 2018

Fingerprint

Inactivated Vaccines
Poliomyelitis
Injections
Vaccines
Vaccination
Antigens
Immunity
Appointments and Schedules
Polymers

Keywords

  • Controlled release
  • Global health
  • Inactivated polio vaccine
  • Single-administration vaccines
  • Vaccine stability

ASJC Scopus subject areas

  • General

Cite this

Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response. / Tzeng, Stephany; McHugh, Kevin J.; Behrens, Adam M.; Rose, Sviatlana; Sugarman, James L.; Ferber, Shiran; Langer, Robert; Jaklenec, Ana.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 23, 05.06.2018, p. E5269-E5278.

Research output: Contribution to journalArticle

Tzeng, Stephany ; McHugh, Kevin J. ; Behrens, Adam M. ; Rose, Sviatlana ; Sugarman, James L. ; Ferber, Shiran ; Langer, Robert ; Jaklenec, Ana. / Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 23. pp. E5269-E5278.
@article{859553cc62cd40ec8210e75bd48f152e,
title = "Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response",
abstract = "Vaccination in the developing world is hampered by limited patient access, which prevents individuals from receiving the multiple injections necessary for protective immunity. Here, we developed an injectable microparticle formulation of the inactivated polio vaccine (IPV) that releases multiple pulses of stable antigen over time. To accomplish this, we established an IPV stabilization strategy using cationic polymers for pH modulation to enhance traditional small-molecule–based stabilization methods. We investigated the mechanism of this strategy and showed that it was broadly applicable to all three antigens in IPV. Our lead formulations released two bursts of IPV 1 month apart, mimicking a typical vaccination schedule in the developing world. One injection of the controlled-release formulations elicited a similar or better neutralizing response in rats, considered the correlate of protection in humans, than multiple injections of liquid vaccine. This single-administration vaccine strategy has the potential to improve vaccine coverage in the developing world.",
keywords = "Controlled release, Global health, Inactivated polio vaccine, Single-administration vaccines, Vaccine stability",
author = "Stephany Tzeng and McHugh, {Kevin J.} and Behrens, {Adam M.} and Sviatlana Rose and Sugarman, {James L.} and Shiran Ferber and Robert Langer and Ana Jaklenec",
year = "2018",
month = "6",
day = "5",
doi = "10.1073/pnas.1720970115",
language = "English (US)",
volume = "115",
pages = "E5269--E5278",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "23",

}

TY - JOUR

T1 - Stabilized single-injection inactivated polio vaccine elicits a strong neutralizing immune response

AU - Tzeng, Stephany

AU - McHugh, Kevin J.

AU - Behrens, Adam M.

AU - Rose, Sviatlana

AU - Sugarman, James L.

AU - Ferber, Shiran

AU - Langer, Robert

AU - Jaklenec, Ana

PY - 2018/6/5

Y1 - 2018/6/5

N2 - Vaccination in the developing world is hampered by limited patient access, which prevents individuals from receiving the multiple injections necessary for protective immunity. Here, we developed an injectable microparticle formulation of the inactivated polio vaccine (IPV) that releases multiple pulses of stable antigen over time. To accomplish this, we established an IPV stabilization strategy using cationic polymers for pH modulation to enhance traditional small-molecule–based stabilization methods. We investigated the mechanism of this strategy and showed that it was broadly applicable to all three antigens in IPV. Our lead formulations released two bursts of IPV 1 month apart, mimicking a typical vaccination schedule in the developing world. One injection of the controlled-release formulations elicited a similar or better neutralizing response in rats, considered the correlate of protection in humans, than multiple injections of liquid vaccine. This single-administration vaccine strategy has the potential to improve vaccine coverage in the developing world.

AB - Vaccination in the developing world is hampered by limited patient access, which prevents individuals from receiving the multiple injections necessary for protective immunity. Here, we developed an injectable microparticle formulation of the inactivated polio vaccine (IPV) that releases multiple pulses of stable antigen over time. To accomplish this, we established an IPV stabilization strategy using cationic polymers for pH modulation to enhance traditional small-molecule–based stabilization methods. We investigated the mechanism of this strategy and showed that it was broadly applicable to all three antigens in IPV. Our lead formulations released two bursts of IPV 1 month apart, mimicking a typical vaccination schedule in the developing world. One injection of the controlled-release formulations elicited a similar or better neutralizing response in rats, considered the correlate of protection in humans, than multiple injections of liquid vaccine. This single-administration vaccine strategy has the potential to improve vaccine coverage in the developing world.

KW - Controlled release

KW - Global health

KW - Inactivated polio vaccine

KW - Single-administration vaccines

KW - Vaccine stability

UR - http://www.scopus.com/inward/record.url?scp=85047967894&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047967894&partnerID=8YFLogxK

U2 - 10.1073/pnas.1720970115

DO - 10.1073/pnas.1720970115

M3 - Article

C2 - 29784798

AN - SCOPUS:85047967894

VL - 115

SP - E5269-E5278

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 23

ER -