Stability of human immunodeficiency virus type 1 antibodies in whole blood dried on filter paper and stored under various tropical conditions in Kinshasa, Zaire

F. Behets, M. Kashamuka, M. Pappaioanou, T. A. Green, R. W. Ryder, V. Batter, J. R. George, W. H. Hannon, T. C. Quinn

Research output: Contribution to journalArticle

Abstract

The use of whole-blood spots on filter paper for the detection of antibody to human immunodeficiency virus type 1 (HIV-1) was evaluated during a 20-week period under a variety of storage environments simulating the harsh tropical field conditions in Kinshasa, Zaire. During the first 6 weeks of storage, all replicates of high- and low-titer HIV-1-positive reference samples remained positive by enzyme immunoassay and Western blotting (immunoblotting), and all replicates of HIV-1-negative samples remained negative under all storage conditions. However, hot and humid storage conditions for up to 20 weeks caused a progressive decline in enzyme immunoassay optical density ratio values, which was particularly noticeable in samples with a low HIV-1 antibody titer. Harsh tropical operational conditions did not cause any repeatedly false-positive results during the 20-week storage period. The use of gas-impermeable bags with desiccant for the storage of blood spots on filter paper improved the stability of HIV-1 antibody detection over time and is recommended for the storage of whole-blood spots on filter paper in harsh tropical field settings.

Original languageEnglish (US)
Pages (from-to)1179-1182
Number of pages4
JournalJournal of clinical microbiology
Volume30
Issue number5
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Stability of human immunodeficiency virus type 1 antibodies in whole blood dried on filter paper and stored under various tropical conditions in Kinshasa, Zaire'. Together they form a unique fingerprint.

  • Cite this

    Behets, F., Kashamuka, M., Pappaioanou, M., Green, T. A., Ryder, R. W., Batter, V., George, J. R., Hannon, W. H., & Quinn, T. C. (1992). Stability of human immunodeficiency virus type 1 antibodies in whole blood dried on filter paper and stored under various tropical conditions in Kinshasa, Zaire. Journal of clinical microbiology, 30(5), 1179-1182.