ST-2, a telomere and subtelomere duplex and G-strand binding protein activity in Trypanosoma brucei

J. E. Eid, B. Sollner-Webb

Research output: Contribution to journalArticle

Abstract

From Trypanosoma brucei, we identified ST-2, a protein complex that interacts with telomeric DNA and exhibits novel features. It binds specifically to the double-stranded telomere repeats (TTAGGG) and more tightly to the subtelomere 29-base pair elements that separate the telomere repeats from their proximal telomere-associated sequences. Interestingly, ST- 2 showed still greater affinity for the G-rich strand of the telomere present either as an overhang or in a single-stranded form, but it exhibited the highest affinity for the G-rich strand of the subtelomere repeats. The binding characteristics of ST-2 are complementary to those of ST-1, a 39-kDa polypeptide we previously identified in T. brucei (Eid, J., and Sollner- Webb, B. (1995) Mol. Cell. Biol. 15, 389-397) that binds preferentially to the C-rich strands of the subtelomere and telomere repeats. UV cross-linking revealed five polypeptides of ST-2 that bind directly to the G-rich strand of the DNA, one of which is phosphorylated. Furthermore, the presence of ST-1 is critical for ST-2 complex binding both to the G-rich strand and to the duplex DNA, evidently as part of the ST-2 complex. This indicates that when binding to the duplex subtelomere and telomere repeats, ST-2 may act as a protein bridge with its ST-1 subunit binding to the C-rich strand and its five other cross-linkable polypeptides binding to the G-rich strand. Such an association could serve to hold the genomic subtelomeric and telomeric sequences in a partially single-stranded configuration to facilitate the recombinational events in this region that are crucial to the parasite.

Original languageEnglish (US)
Pages (from-to)14927-14936
Number of pages10
JournalJournal of Biological Chemistry
Volume272
Issue number23
DOIs
StatePublished - 1997

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Trypanosoma brucei brucei
Telomere
Carrier Proteins
Peptides
DNA
Proteins
Base Pairing
Parasites

ASJC Scopus subject areas

  • Biochemistry

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ST-2, a telomere and subtelomere duplex and G-strand binding protein activity in Trypanosoma brucei. / Eid, J. E.; Sollner-Webb, B.

In: Journal of Biological Chemistry, Vol. 272, No. 23, 1997, p. 14927-14936.

Research output: Contribution to journalArticle

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