SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae

Catherine Dollard, Stephanie L. Ricupero-Hovasse, Georges Natsoulis, Jef D. Boeke, Fred Winston

Research output: Contribution to journalArticle

Abstract

The Saccharomyces cerevisiae genome contains four loci that encode histone proteins. Two of these loci, HTA1-HTB1 and HTA2-HTB2, each encode histones H2A and H2B. The other two loci, HHT1-HHF1 and HHT2-HHF2, each encode histones H3 and H4. Because of their redundancy, deletion of any one histone locus does not cause lethality. Previous experiments demonstrated that mutations at one histone locus, HTA1-HTB1, do cause lethality when in conjunction with mutations in the SPT10 gene. SPT10 has been shown to be required for normal levels of transcription of several genes in S. cerevisiae. Motivated by this double-mutant lethality, we have now investigated the interactions of mutations in SPT10 and in a functionally related gene, SPT21, with mutations at each of the four histone loci. These experiments have demonstrated that both SPT10 and SPT21 are required for transcription at two particular histone loci, HTA2-HTB2 and HHF2-HHT2, but not at the other two histone loci. These results suggest that under some conditions, S. cerevisiae may control the level of histone proteins by differential expression of its histone genes.

Original languageEnglish (US)
Pages (from-to)5223-5228
Number of pages6
JournalMolecular and Cellular Biology
Volume14
Issue number8
StatePublished - Aug 1994

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Histones
Saccharomyces cerevisiae
Genes
Mutation
Proteins
Genome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Dollard, C., Ricupero-Hovasse, S. L., Natsoulis, G., Boeke, J. D., & Winston, F. (1994). SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae. Molecular and Cellular Biology, 14(8), 5223-5228.

SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae. / Dollard, Catherine; Ricupero-Hovasse, Stephanie L.; Natsoulis, Georges; Boeke, Jef D.; Winston, Fred.

In: Molecular and Cellular Biology, Vol. 14, No. 8, 08.1994, p. 5223-5228.

Research output: Contribution to journalArticle

Dollard, C, Ricupero-Hovasse, SL, Natsoulis, G, Boeke, JD & Winston, F 1994, 'SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae', Molecular and Cellular Biology, vol. 14, no. 8, pp. 5223-5228.
Dollard C, Ricupero-Hovasse SL, Natsoulis G, Boeke JD, Winston F. SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae. Molecular and Cellular Biology. 1994 Aug;14(8):5223-5228.
Dollard, Catherine ; Ricupero-Hovasse, Stephanie L. ; Natsoulis, Georges ; Boeke, Jef D. ; Winston, Fred. / SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae. In: Molecular and Cellular Biology. 1994 ; Vol. 14, No. 8. pp. 5223-5228.
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