Sprouty proteins inhibit receptor-mediated activation of phosphatidylinositol-specific phospholipase C

Simge Akbulut, Alagarsamy L. Reddi, Priya Aggarwal, Charuta Ambardekar, Barbara Canciani, Marianne K H Kim, Laura Hix, Tomas Vilimas, Jacqueline Mason, M. Albert Basson, Matthew Lovatt, Jonathan Powell, Samuel Lester Collins, Steven Quatela, Mark Phillips, Jonathan D. Licht

Research output: Contribution to journalArticle

Abstract

Sprouty (Spry) proteins are negative regulators of receptor tyrosine kinase signaling; however, their exact mechanism of action remains incompletely understood. We identified phosphatidylinositol-specific phospholipase C (PLC)-γ as a partner of the Spry1 and Spry2 proteins. Spry-PLCγ interaction was dependent on the Src homology 2 domain of PLCγ and a conserved N-terminal tyrosine residue in Spry1 and Spry2. Overexpression of Spry1 and Spry2 was associated with decreased PLCγ phosphorylation and decreased PLCγ activity as measured by production of inositol (1,4,5)-triphosphate (IP3) and diacylglycerol, whereas cells deficient for Spry1 or Spry1, -2, and -4 showed increased production of IP3 at baseline and further increased in response to growth factor signals. Overexpression of Spry 1 or Spry2 or small-interfering RNA-mediated knockdown of PLCγ1 or PLCγ2 abrogated the activity of a calcium-dependent reporter gene, suggesting that Spry inhibited calcium-mediated signaling downstream of PLCγ. Furthermore, Spry overexpression in T-cells, which are highly dependent on PLCγ activity and calcium signaling, blocked T-cell receptor-mediated calcium release. Accordingly, cultured T-cells from Spry1 gene knockout mice showed increased proliferation in response to T-cell receptor stimulation. These data highlight an important action of Spry, which may allow these proteins to influence signaling through multiple receptors.

Original languageEnglish (US)
Pages (from-to)3487-3496
Number of pages10
JournalMolecular Biology of the Cell
Volume21
Issue number19
DOIs
StatePublished - Oct 1 2010

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Phosphoinositide Phospholipase C
Type C Phospholipases
Proteins
Calcium Signaling
T-Cell Antigen Receptor
Calcium
T-Lymphocytes
Gene Knockout Techniques
Inositol 1,4,5-Trisphosphate
src Homology Domains
Diglycerides
Receptor Protein-Tyrosine Kinases
Reporter Genes
Knockout Mice
Small Interfering RNA
Tyrosine
Cultured Cells
Intercellular Signaling Peptides and Proteins
Phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Akbulut, S., Reddi, A. L., Aggarwal, P., Ambardekar, C., Canciani, B., Kim, M. K. H., ... Licht, J. D. (2010). Sprouty proteins inhibit receptor-mediated activation of phosphatidylinositol-specific phospholipase C. Molecular Biology of the Cell, 21(19), 3487-3496. https://doi.org/10.1091/mbc.E10-02-0123

Sprouty proteins inhibit receptor-mediated activation of phosphatidylinositol-specific phospholipase C. / Akbulut, Simge; Reddi, Alagarsamy L.; Aggarwal, Priya; Ambardekar, Charuta; Canciani, Barbara; Kim, Marianne K H; Hix, Laura; Vilimas, Tomas; Mason, Jacqueline; Basson, M. Albert; Lovatt, Matthew; Powell, Jonathan; Collins, Samuel Lester; Quatela, Steven; Phillips, Mark; Licht, Jonathan D.

In: Molecular Biology of the Cell, Vol. 21, No. 19, 01.10.2010, p. 3487-3496.

Research output: Contribution to journalArticle

Akbulut, S, Reddi, AL, Aggarwal, P, Ambardekar, C, Canciani, B, Kim, MKH, Hix, L, Vilimas, T, Mason, J, Basson, MA, Lovatt, M, Powell, J, Collins, SL, Quatela, S, Phillips, M & Licht, JD 2010, 'Sprouty proteins inhibit receptor-mediated activation of phosphatidylinositol-specific phospholipase C', Molecular Biology of the Cell, vol. 21, no. 19, pp. 3487-3496. https://doi.org/10.1091/mbc.E10-02-0123
Akbulut, Simge ; Reddi, Alagarsamy L. ; Aggarwal, Priya ; Ambardekar, Charuta ; Canciani, Barbara ; Kim, Marianne K H ; Hix, Laura ; Vilimas, Tomas ; Mason, Jacqueline ; Basson, M. Albert ; Lovatt, Matthew ; Powell, Jonathan ; Collins, Samuel Lester ; Quatela, Steven ; Phillips, Mark ; Licht, Jonathan D. / Sprouty proteins inhibit receptor-mediated activation of phosphatidylinositol-specific phospholipase C. In: Molecular Biology of the Cell. 2010 ; Vol. 21, No. 19. pp. 3487-3496.
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AU - Canciani, Barbara

AU - Kim, Marianne K H

AU - Hix, Laura

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AU - Lovatt, Matthew

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