Abstract
Methylation of DNA in CpG dense regions of gene promoters (CpG islands) is important for transcriptional inactivation of selective genes in normal and neoplastic cells. Here, we presents a spreadsheet-based program adapted from Microsoft® Excel® that is useful for identifying CpG islands and for assisting in the laboratory analysis of DNA methylation of these regions. Upon execution of the program, a customized workbook analyzes an entered DNA sequence for the total number and percentage cytosine and guanine nucleotides, the total number and percentage of CpG sites, and a CpG:GpC ratio. The program also displays the distribution of CpG sites in a visual format as well as in two different graphical formats. Finally, the program assists in laboratory studies of DNA methylation that employ bisulfite modification of DNA by displaying methylation-dependent effects of bisulfite treatment on DNA sequences.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 110-114 |
| Number of pages | 5 |
| Journal | BioTechniques |
| Volume | 30 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2001 |
ASJC Scopus subject areas
- Biotechnology
- General Biochemistry, Genetics and Molecular Biology