Spore coat architecture of Clostridium novyi NT spores

Marco Plomp, J. Michael McCaffery, Ian Cheong, Xin Huang, Chetan Bettegowda, Kenneth W Kinzler, Shibin Zhou, Bert Vogelstein, Alexander J. Malkin

Research output: Contribution to journalArticle

Abstract

Spores of the anaerobic bacterium Clostridium novyi NT are able to germinate in and destroy hypoxic regions of tumors in experimental animals. Future progress in this area will benefit from a better understanding of the germination and outgrowth processes that are essential for the tumorilytic properties of these spores. Toward this end, we have used both transmission electron microscopy and atomic force microscopy to determine the structure of both dormant and germinating spores. We found that the spores are surrounded by an amorphous layer intertwined with honeycomb parasporal layers. Moreover, the spore coat layers had apparently self-assembled, and this assembly was likely to be governed by crystal growth principles. During germination and outgrowth, the honeycomb layers, as well as the underlying spore coat and undercoat layers, sequentially dissolved until the vegetative cell was released. In addition to their implications for understanding the biology of C. novyi NT, these studies document the presence of proteinaceous growth spirals in a biological organism.

Original languageEnglish (US)
Pages (from-to)6457-6468
Number of pages12
JournalJournal of Bacteriology
Volume189
Issue number17
DOIs
StatePublished - Sep 2007

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Clostridium
Spores
Germination
Anaerobic Bacteria
Atomic Force Microscopy
Crystallization
Transmission Electron Microscopy
Growth
Neoplasms

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Plomp, M., McCaffery, J. M., Cheong, I., Huang, X., Bettegowda, C., Kinzler, K. W., ... Malkin, A. J. (2007). Spore coat architecture of Clostridium novyi NT spores. Journal of Bacteriology, 189(17), 6457-6468. https://doi.org/10.1128/JB.00757-07

Spore coat architecture of Clostridium novyi NT spores. / Plomp, Marco; McCaffery, J. Michael; Cheong, Ian; Huang, Xin; Bettegowda, Chetan; Kinzler, Kenneth W; Zhou, Shibin; Vogelstein, Bert; Malkin, Alexander J.

In: Journal of Bacteriology, Vol. 189, No. 17, 09.2007, p. 6457-6468.

Research output: Contribution to journalArticle

Plomp, Marco ; McCaffery, J. Michael ; Cheong, Ian ; Huang, Xin ; Bettegowda, Chetan ; Kinzler, Kenneth W ; Zhou, Shibin ; Vogelstein, Bert ; Malkin, Alexander J. / Spore coat architecture of Clostridium novyi NT spores. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 17. pp. 6457-6468.
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