Spontaneous mutation rate to thioguanine resistance is decreased in polyploid hamster cells

John Morrow, Douglas Stocco, Emily Barron

Research output: Contribution to journalArticle

Abstract

The mutation rate to thioguanine resistance was 3.11 × 10−6 in a near diploid V79 hamster cell line and 7.58 × 10−8 in a near tetraploid derivative produced with colchicine. The specific activities of glucose‐6‐phosphate dehydrogenase and phosphoglycerate kinase of the tetraploid line were greater than that of the diploid which suggests that twice the number of active X chromosomes were present in the tetraploid. These results are compatible with the hypothesis that spontaneous variants resistant to thioguanine arise through mutation and chromosomal segregation, as has been suggested for induced mutations in tetraploid hamster cells.

Original languageEnglish (US)
Pages (from-to)81-85
Number of pages5
JournalJournal of Cellular Physiology
Volume96
Issue number1
DOIs
StatePublished - Jul 1978
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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