Spontaneous Degranulation of Cultured Bone-Marrow-Derived Immature Mast Cells from X-Linked Immunodeficient (Xid) Mice

Ann M. Dvorak, Toru Miura, Linda Letourneau, Teruko Ishizaka, Toshiaki Kawakami

Research output: Contribution to journalArticle

Abstract

IgE-mediated, antigen-dependent stimulation of immature mouse mast cells cultured in IL-3-containing media produces secretion by granule exocytosis. Similar cultured mast cells were derived from X-linked immunodeficient (Xid) mice and examined by electron microscopy. In these cultures, Xid mast cells were also immature. In contrast to cultures obtained from control mice, 10-20% of the immature mast cells of Xid origin were undergoing secretion by granule extrusion in the absence of any secretogogue. Spontaneous secretion may be related to discordered tyrosine kinase function and/or signal transduction pathways in the Xid mouse.

Original languageEnglish (US)
Pages (from-to)118-125
Number of pages8
JournalInternational Archives of Allergy and Immunology
Volume111
Issue number2
DOIs
StatePublished - Jan 1 1996

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Keywords

  • Degranulation
  • Mast cell
  • Secretion
  • Xid mice

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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