Spontaneous cell polarization through actomyosin-based delivery of the Cdc42 GTPase

Roland Wedlich-Soldner, Steve Altschuter, Lani Wu, Rong Li

Research output: Contribution to journalArticle

Abstract

Cell polarization can occur in the absence of any spatial cues. To investigate the mechanism of spontaneous cell polarization, we used an assay in yeast where expression of an activated form of Cdc42, a Rho-type guanosine triphosphatase (GTPase) required for cell polarization, could generate cell polarity without any recourse to a preestablished physical cue. The polar distribution of Cdc42 in this assay required-targeted secretion directed by the actin cytoskeleton. A mathematical simulation showed that a stable polarity axis could be generated through a positive feedback loop in which a stochastic increase in the local concentration of activated Cdc42 on the plasma membrane enhanced the probability of actin polymerization and increased the probability of further Cdc42 accumulation to that site.

Original languageEnglish (US)
Pages (from-to)1231-1235
Number of pages5
JournalScience
Volume299
Issue number5610
DOIs
StatePublished - Feb 21 2003

ASJC Scopus subject areas

  • General

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